| 产品编号 | bs-10376R |
| 英文名称 | Orexin A |
| 中文名称 | 增食欲素A/欲激素A抗体 |
| 别 名 | HCRT 1; HCRT; HCRT1; HCRT2; Hypocretin (orexin) neuropeptide precursor; Hypocretin 1; Hypocretin 2; Hypocretin A; Hypocretin; Hypocretin neuropeptide precursor; Hypocretin1; NRCLP1; Orexin A; Orexin; Orexin B; Orexin precursor; OrexinA; OX; PPORX; PPOX; PREPROOREXIN; OREX_HUMAN. |
| 研究领域 | 生长因子和激素 内分泌病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 3.6kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Orexin A: 1-100/131 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Orexin A (hypocretin 1) a 33 amino acid peptide and orexin B (hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Orexin A and orexin B bind to and activate two closely related G protein coupled receptors (GPCRs), termed Orexin 1 (OX1) and Orexin 2 (OX2) receptors. Dysfunction of the orexin peptide system has been linked to narcolepsy. Function: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. Subcellular Location: Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse. Note=Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. Tissue Specificity: Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas. Post-translational modifications: Specific enzymatic cleavages at paired basic residues yield the different active peptides. DISEASE: Defects in HCRT are the cause of narcolepsy type 1 (NRCLP1) [MIM:161400]. Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed. Note=Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Similarity: Belongs to the orexin family. SWISS: O43612 Gene ID: 3060 Database links: Entrez Gene: 3060 Human Omim: 602358 Human SwissProt: O43612 Human Unigene: 158348 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
