| 产品编号 | bs-15470R |
| 英文名称 | HEXO Rabbit pAb |
| 中文名称 | 3'-5'外切酶1蛋白抗体 |
| 别 名 | ERI1; HEXO; THEX1; 3'HEXO; ERI1 exoribonuclease 1; 3' 5' exonuclease ERI 1; 3' 5' exonuclease ERI1; 3' EXO; 3' exoribonuclease; 3' HEXO; 3' histone mRNA exonuclease 1; 3''-5'' exonuclease eri1; 3''-5'' exoribonuclease 1; 3'EXO; 3'HEXO; ERI 1; Eri 1 homolog; Eri-1 homolog; eri1; Eri1 homolog; ERI1_HUMAN; HEXO; Histone mRNA 3' end specific exonuclease; Histone mRNA 3' end specific exoribonuclease; Histone mRNA 3''-end-specific exoribonuclease; Histone mRNA 3''-exonuclease 1; MGC35395; Protein 3''hExo; THEX 1; Three prime histone mRNA exonuclease 1. |
| 研究领域 | 染色质和核信号 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HEXO: 51-150/349 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Helicase with RNase motif, more commonly designated Dicer, cleaves double-stranded RNA (dsRNA) in the RNA interference and small temporal RNA (stRNA) pathways, producing active small RNA components (siRNAs) which target the destruction of RNA and repress gene expression. Human Dicer cleaves dsRNA independent of ATP. The 3’-5’ exonuclease ERI-1, also known as Protein 3'hExo, degrades Histone mRNA after replication and may be involved in the regulation of RNA interference. ERI-1 has a high affinity for the stem-loop structure of replication-dependent Histone pre-mRNAs. It requires the 5'-ACCCA-3' sequence present in stem-loop structure. ERI-1 and a stem-loop binding protein (SLBP) target opposite faces of a unique highly conserved stem-loop RNA scaffold towards the 3' end of Histone mRNA. Function: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs. Subunit: Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1. Interacts in a cooperative manner with SLBP to the mature 3'-end of histone mRNAs (By similarity). Binds to 40S and 60S ribosomal subunits and to 80S assembled ribosomes. Found in a ternary complex with SLBP and the stem-loop structure of the 3'-end of histone mRNAs. Subcellular Location: Cytoplasm. Nucleus. Nucleus, nucleolus. Similarity: Contains 1 exonuclease domain. Contains 1 SAP domain. SWISS: Q8IV48 Gene ID: 90459 Database links: Entrez Gene: 90459 Human Omim: 608739 Human SwissProt: Q8IV48 Human Unigene: 20000 Human Unigene: 17469 Rat |
| 产品图片 |
Tissue/cell: mouse testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HEXO Polyclonal Antibody, Unconjugated(bs-15470R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
