| 产品编号 | bs-15453R |
| 英文名称 | HEMK2 Rabbit pAb |
| 中文名称 | Hemk甲基转移酶家族2号蛋白抗体 |
| 别 名 | C21orf127; Chromosome 21 open reading frame 127; EC 2.1.1.-; HemK methyltransferase family member 2; HemK2; M.HsaHemK2P; MGC19995; MTQ2; N-6 adenine-specific DNA methyltransferase 1; N-6 adenine-specific DNA methyltransferase 1(putative); N6-DNA-methyltransferase; N6AMT; N6AMT1; OTTHUMP00000096393; OTTHUMP00000096394; PRED28; HEMK2_HUMAN. |
| 研究领域 | 细胞生物 免疫学 染色质和核信号 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Sheep,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HEMK2: 1-100/214 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
HEMK2 is a 214 amino acid protein that belongs to the methyltransferase superfamily and exists as multiple alternatively spliced isoforms. The gene encoding HEMK2 maps to human chromosome 21, which houses approximately 300 genes and comprises nearly 1.5% of the human genome. Chromosome 21-associated disorders include Alzheimer's disease, amyotrophic lateral sclerosis and, most notably, Down syndrome (also known as trisomy 21). Function: Heterodimeric methyltransferase that catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated. May play a role in the modulation of arsenic-induced toxicity. May be involved in the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid. Subunit: Heterodimer with TRMT112. Tissue Specificity: Widely expressed, with highest expression in parathyroid and pituitary glands, followed by adrenal gland and kidney, and lowest expression in leukocytes and mammary gland. Similarity: Belongs to the eukaryotic/archaeal PrmC-related family. SWISS: Q9Y5N5 Gene ID: 29104 Database links: Entrez Gene: 29104 Human Omim: 614553 Human SwissProt: Q9Y5N5 Human Unigene: 163846 Human |
| 产品图片 |
Sample: A549 Cell (Human) Lysate at 30 ug
Primary: Anti-HEMK2 (bs-15453R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23kD
Observed band size: 23kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
