| 产品编号 | bs-15450R |
| 英文名称 | HELZ Rabbit pAb |
| 中文名称 | 人肿瘤抑制蛋白抗体 |
| 别 名 | DKFZp586G1924; Down regulated in human cancers; DRHC; Helicase with zinc finger; HUMORF 5; HUMORF5; KIAA0054; MGC163454; Probable helicase with zinc finger domain; HELZ_HUMAN. |
| 研究领域 | 细胞生物 免疫学 染色质和核信号 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 219 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HELZ: 1-100/1942 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Helicases comprise a class of enzymes that function as motor proteins which move along nucleic acid phosphodiester bonds, effectively separating two annealed nucleic acid strands. RNA helicases alter the conformation of RNA, specifically by unwinding double-stranded RNA regions to yield single RNA strands, a process which changes the biological activity of the RNA molecule. HELZ (helicase with zinc finger), also known as DHRC or HUMORF5, is a 1,942 amino acid nuclear protein that contains one C3H1-type zinc finger and belongs to the RNA helicase superfamily. Expressed ubiquitously during embryonic development, HELZ is thought to function as an RNA helicase that modifies RNA structure and plays a role in the development of multiple organs and tissues within the developing embryo. Function: May act as a helicase that plays a role in RNA metabolism in multiple tissues and organs within the developing embryo. Subunit: Interacts with SMYD2 (By similarity). Interacts with POLR2A. Interacts with SMYD3; the interaction may bridge SMYD3 and RNA polymerase II. Subcellular Location: Nucleus (Probable). Tissue Specificity: Expressed predominantly in thymus and brain. Expression is down-regulated in 28 of 95 tested cancer cell lines. Similarity: Belongs to the DNA2/NAM7 helicase family. Contains 1 C3H1-type zinc finger. SWISS: P42694 Gene ID: 9931 Database links: Entrez Gene: 9931 Human Entrez Gene: 78455 Mouse Omim: 606699 Human SwissProt: P42694 Human SwissProt: Q6DFV5 Mouse |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HELZ Polyclonal Antibody, Unconjugated(bs-15450R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
