| 产品编号 | bs-15434R |
| 英文名称 | HDPTP Rabbit pAb |
| 中文名称 | His 结构域内含酪氨酸磷酸酶蛋白抗体 |
| 别 名 | HD-PTP; HDPTP; NEDBASS; PTP-TD14; Q8N6Z5_HUMAN; PTN23_HUMAN; PTPN23; His domain-containing protein tyrosine phosphatase (HD-PTP); Protein tyrosine phosphatase TD14 (PTP-TD14); 3.1.3.48; KIAA1471; PTN23_MOUSE; Q8R1Z5_MOUSE; PTN23_RAT; Q9QZP8_RAT; |
| 研究领域 | 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Chicken) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 179 kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HDPTP: 1501-1636/1636 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
HD-PTP is a 1,636 amino acid protein encoded by the human gene PTPN23. HD-PTP belongs to the protein-tyrosine phosphatase family, non-receptor class subfamily. It contains one BRO1 domain, two TPR repeats and one tyrosine-protein phosphatase domain. The C-terminal region contains the PTP-like domain, whereas the N-terminal region contains the two TPR regions. These regions are homologous to the yeast protein, BRO1, which is involved in the mitogen-activated protein kinase signaling pathway. Similarly, HD-PTP is believed to act as a negative regulator of Ras-mediated mitogenic activity and is phosphorylated upon DNA damage, probably by ATM or ATR. HD-PTP protein is differentially modulated by two angiogenic growth factors. While Vascular Endothelial Growth Factor (VEGF) has no affect on protein levels, Fibroblast Growth Factor-2 (FGF-2) induces HD-PTP degradation via the proteasome system. Function: May act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis. Subunit: Interacts with GRAP2 and GRB2. Subcellular Location: Nucleus. Cytoplasm. Cytoplasmic vesicle. Endosome. Cytoplasm, cytoskeleton, cilium basal body. Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 BRO1 domain. Contains 2 TPR repeats. Contains 1 tyrosine-protein phosphatase domain. SWISS: Q9H3S7 Gene ID: 25930 Database links: Entrez Gene: 25930 Human Entrez Gene: 104831 Mouse Omim: 606584 Human SwissProt: Q8N6Z5 Human SwissProt: Q9H3S7 Human SwissProt: Q6PB44 Mouse SwissProt: Q8R1Z5 Mouse Unigene: 25524 Human Unigene: 123884 Mouse Unigene: 335477 Mouse Unigene: 54442 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HDPTP) Polyclonal Antibody, Unconjugated (bs-15434R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HDPTP) Polyclonal Antibody, Unconjugated (bs-15434R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
