| 产品编号 | bs-15410R |
| 英文名称 | HAPLN1 |
| 中文名称 | 透明质酸及粘蛋白1抗体 |
| 别 名 | Cartilage link protein; cartilage linking protein 1; CLP; CRTL1; Crtl1l; hyaluronan and proteoglycan link protein 1; Hyaluronan and proteoglycan link protein 1 precursor; LP; Proteoglycan link protein; HPLN1_HUMAN. |
| 研究领域 | 信号转导 细菌及病毒 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Dog,Pig,Cow,Horse,Sheep) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HAPLN1 : 21-120/354 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The human cartilage link protein, also designated HAPLN1, is a member of the hyaluronan and proteoglycan link protein (HAPLN) family of proteins. This family contains 4 proteins with approximately 50% homology. The human cartilage link protein strengthens tissue architecture by using hyaluronic acid to stabilize the aggregates of proteoglycan monomers inside the extracellular cartilage matrix. It is widely expressed in most tissue types except brain tissue where, much like the other human cartilage link protein genes, it is weakly expressed. The HAPLN and the brain-specific CSPG genes, as well as other members of the link module superfamily, appear to have a common ancestral gene origin. Function: Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Widely expressed. Weakly expressed in the brain. Similarity: Belongs to the HAPLN family. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Contains 2 Link domains. SWISS: P10915 Gene ID: 1404 Database links: Entrez Gene: 1404 Human Entrez Gene: 12950 Mouse Omim: 115435 Human SwissProt: P10915 Human SwissProt: Q9QUP5 Mouse Unigene: 2799 Human Unigene: 266790 Mouse Unigene: 50531 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HAPLN1) Polyclonal Antibody, Unconjugated (bs-15410R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
