| 产品编号 | bs-15404R |
| 英文名称 | Rabbit Anti-HAGH antibody |
| 中文名称 | 羟酰谷胱甘肽水解酶蛋白抗体 |
| 别 名 | GLO2; GLX II; Glyoxalase II; HAGH; HAGH1; Hydroxyacyl glutathione hydrolase; Hydroxyacylglutathione hydrolase, mitochondrial; GLO2_HUMAN. |
| 研究领域 | 细胞生物 免疫学 神经生物学 新陈代谢 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 32kDa |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HAGH: 221-308/308 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The glyoxal pathway plays a role in the detoxification of glucose degradation products (GDP). Glyoxalase I and Gyloxalase II (also designated hydroxyacyl glutathione hydrolase or HAGH) are members of the Gyloxalase family. The Gyloxalase II enzyme is a thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form reduced glutathione and D-lactic acid. It exists only as a monomer and binds two zinc ions per subunit. Glyoxalase II contains 260 amino acids. It is detected in the mitochondria and cytosol of mammals. Both Glyoxalase I and Gyloxalase II are detected at a higher activity level in breast cancer tissues than with matched unaffected tissues. This suggests that glyoxalase inhibitor drugs may be effective in the treatment of cancer. Function: Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid. Subunit: Monomer. Subcellular Location: Isoform 1: Mitochondrion matrix. Isoform 2: Cytoplasm. Tissue Specificity: Expressed in liver and kidney. Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. SWISS: Q16775 Gene ID: 3029 Database links: Entrez Gene: 3029 Human Entrez Gene: 14651 Mouse Omim: 138760 Human SwissProt: Q16775 Human SwissProt: Q99KB8 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
