| 产品编号 | bs-15366R |
| 英文名称 | Rabbit Anti-GPR92 antibody |
| 中文名称 | G蛋白偶联受体GPR92蛋白抗体 |
| 别 名 | GPCR GPR92; G-protein coupled receptor 92; G-protein coupled receptor 93; GPR92; GPR93; KPG_010; LPA receptor 5; LPA-5; LPA5; LPAR5; Lysophosphatidic acid receptor 5; LPAR5_HUMAN; GPCR 92. |
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Specific References (4) | bs-15366R has been referenced in 4 publications.
[IF=9.77] Tang Y et al. PARP14 inhibits microglial activation via LPAR5 to promote post-stroke functional recoveryAutophagy.2020 Dec 15;1-18. WB ; Mouse.
[IF=2.942] Xue Gang. et al. Identification of key genes of papillary thyroid carcinoma by integrated bioinformatics analysis. Bioscience Rep. 2020 Aug;40(8):BSR20201555 WB ; Human.
[IF=2.903] Xuling Zhang. et al. Effects of lysophosphatidic acid receptor 5 on NLRC4 inflammasome in brain tissues of transient cerebral ischemia/reperfusion rat:. Hum Exp Toxicol. 2022;(): IF ; Rat.
[IF=2.311] Bingsheng Yang. et al. Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma. Oncol Lett. 2021 Mar;21(3):1-1 IHC ; Human.
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| 研究领域 | 细胞生物 免疫学 信号转导 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 41kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR92: 121-220/372 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the rhodopsin class of G protein-coupled transmembrane receptors. This protein transmits extracellular signals from lysophosphatidic acid to cells through heterotrimeric G proteins and mediates numerous cellular processes. Many G protein receptors serve as targets for pharmaceutical drugs. Transcript variants of this gene have been described.[provided by RefSeq, Dec 2008] Function: Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Not expressed in frontal cortex, basal forebrain, caudate putamen, thalamus, or hippocampus. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q9H1C0 Gene ID: 57121 Database links: Entrez Gene: 57121 Human Omim: 606926 Human SwissProt: Q9H1C0 Human Unigene: 155538 Human |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Human K562 cell lysates
Lane 4: Human A431 cell lysates
Lane 5: Human Jurkat cell lysates
Primary: Anti-GPR92 (bs-15366R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 46 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR92 Polyclonal Antibody, Unconjugated(bs-15366R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR92 Polyclonal Antibody, Unconjugated(bs-15366R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
