| 产品编号 | bs-13554R |
| 英文名称 | Rabbit Anti-ZBED4 antibody |
| 中文名称 | 锌指蛋白BED4抗体 |
| 别 名 | Zinc finger BED domain containing 4; Zinc finger BED domain containing protein 4; Zinc finger BED type containing 4; ZBED4_HUMAN. |
| 研究领域 | 细胞生物 信号转导 锌指蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 130kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZBED4: 101-200/1171 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The zinc finger BED domain-containing protein family (ZBED) is comprised of ZBED1, ZBED2, ZBED3, ZBED4 and ZBED5. They each contain one BED-type zinc finger domains with the exception of ZBED4, which contain 4 BED-type zinc finger domains. ZBED1 is thought to function as a transcription factor that regulates a number of ribosomal protein (RP) encoded genes by binding specifically to 5'-TGTCG[CT]GA[CT]A-3' DNA regions found in RP promotors. ZBED3 is an Axin-binding protein involved in Wnt/beta-catenin signaling modulation. ZBED4 expression has been shown in human and mouse retinas where it is thought to act as a regulatory protein in cone photoreceptors and Müller cells. The functions of ZBED2 and ZBED5 have yet to be elucidated. Subunit: Homodimer. Subcellular Location: Cytoplasmic and Nuclear Tissue Specificity: Widely expressed with highest levels in testis, kidney and spinal cord and brain corpus callosum. Expressed in the retina, found in the cone photoreceptors, Mueller cells, cone pedicles and in the innermost retinal layer. Similarity: Contains 4 BED-type zinc fingers. SWISS: O75132 Gene ID: 9889 Database links: Entrez Gene: 9889 Human Omim: 612552 Human SwissProt: O75132 Human Unigene: 475208 Human |
| 产品图片 |
Sample: U251 (human)cell Lysate at 40 ug
Primary: Anti- ZBED4 (bs-13554R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 135 kD
Sample: U2os (human)cell Lysate at 40 ug
Primary: Anti- ZBED4 (bs-13554R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
