| 产品编号 | bs-13544R |
| 英文名称 | Guanylyl Cyclase alpha 1 Rabbit pAb |
| 中文名称 | 鸟苷酸环化酶α1/GCS-α-1抗体 |
| 别 名 | GC S alpha 1; GC SA3; GCS alpha 1; GCS alpha 3; GCS-alpha-1; GCS-alpha-3; GCYA3_HUMAN; Guanylate cyclase 1 soluble alpha 3; Guanylate cyclase soluble subunit alpha 1; Guanylate cyclase soluble subunit alpha 3; Guanylate cyclase soluble subunit alpha-3; Guc1a1; GUC1A3; GUCA3; GUCSA3; Gucy1a1; Gucy1a3; Soluble guanylate cyclase large subunit; α1 Soluble Guanylyl Cyclase; sGCα1. |
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Specific References (2) | bs-13544R has been referenced in 2 publications.
[IF=11.15] Liu, Yiwei. et al. An electrochemical nitric oxide generator for in-home inhalation therapy in pulmonary artery hypertension. BMC MED. 2022 Dec;20(1):1-13 WB ; Pig.
[IF=3.69] Rui Chen. et al. Tongmai Yangxin pill reduces myocardial No-reflow via endothelium-dependent NO-cGMP signaling by activation of the cAMP/PKA pathway. J Ethnopharmacol. 2021 Mar;267:113462 WB ; Rat.
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| 研究领域 | 细胞生物 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 77 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Guanylyl Cyclase alpha 1: 301-400/690 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase. The soluble forms, known as GCS or sGC, act as receptors for nitric oxide. The membrane-bound receptor forms, known as GC, are peptide hormone receptors. GCS, a cGMP-synthesizing enzyme, is the major receptor for the neurotransmitter nitric oxide (NO). It plays a crucial role in smooth muscle contractility, platelet reactivity and neurotransmission. GCS is a heme containing heterodimer, consisting of one alpha subunit, designated GCS-alpha-1, and one beta subunit. The heme moeity mediates NO activation, and this heme group also binds carbon monoxide, which weakly stimulates the enzyme. Both NO and CO stimulation are enhanced by the allosteric activator 3-(5'-hydroxymethyl-2'furyl)-benzyl-indazole, YC-1. YC-1 can also stimulate GCS in a NO-independent manner. Both the alpha and beta subunits are required for cGMP generation, and at least two isoforms exist for each subunit. Heterodimers consisting of alpha-1/beta-1 and alpha-2/beta-1 have been identified, and both display similar enzymatic activity. Subunit: Heterodimer of an alpha and a beta chain. Subcellular Location: Cytoplasm. Similarity: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 1 guanylate cyclase domain. SWISS: Q02108 Gene ID: 2982 Database links: Entrez Gene: 2982 Human Entrez Gene: 60596 Mouse Omim: 139396 Human SwissProt: Q02108 Human SwissProt: Q9ERL9 Mouse Unigene: 24258 Human Unigene: 143831 Mouse Unigene: 1974 Rat |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GCS alpha 1 Polyclonal Antibody, Unconjugated(bs-13544R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
