产品中心-北京博奥森生物技术有限公司

产品编号	bs-8878R
英文名称	LC3
中文名称	自噬微管相关蛋白轻链3抗体
别名	Autophagy related protein LC3 A; Autophagy related protein LC3 B; Autophagy related ubiquitin like modifier LC3 A; Autophagy related ubiquitin like modifier LC3 B; MAP1 light chain 3 like protein 1; MAP1 light chain 3 like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1ALC3; MAP1BLC3; MAP1LC3A; MAP1LC3B; Microtubule associated protein 1 light chain 3 alpha; Microtubule associated protein 1 light chain 3 beta; Microtubule associated proteins 1A/1B light chain 3; Microtubule associated proteins 1A/1B light chain 3A; Microtubule associated proteins 1A/1B light chain 3B; MLP3A_HUMAN.
	Specific References (29) \| bs-8878R has been referenced in 29 publications. [IF=10.753] Zhuo Ma. et al. Manganese-induced α-synuclein overexpression promotes the accumulation of dysfunctional synaptic vesicles and hippocampal synaptotoxicity by suppressing Rab26-dependent autophagy in presynaptic neurons. SCI TOTAL ENVIRON. 2023 Feb;858:159753 IF ; Mouse. PubMed:36341850 [IF=7.31] Yi-Jing Yang. et al. Lycium barbarum Polysaccharides Regulating miR-181/Bcl-2 Decreased Autophagy of Retinal Pigment Epithelium with Oxidative Stress. OXID MED CELL LONGEV. 2023 Jan 05;2023:9554457 WB ; Mouse. PubMed:36644575 [IF=6.814] Guo-Jian Jiang. et al. Ultraviolet B irradiation induces senescence of human corneal endothelial cells in vitro by DNA damage response and oxidative stress. J PHOTOCH PHOTOBIO B. 2022 Oct;235:112568 WB ; Human. PubMed:36137302 [IF=6.551] Yukun Fang. et al. Activation of the ROS/HO-1/NQO1 signaling pathway contributes to the copper-induced oxidative stress and autophagy in duck renal tubular epithelial cells. Sci Total Environ. 2021 Feb;757:143753 WB ; Duck. PubMed:33316526 [IF=5.811] Lang Xiu-Yuan. et al. Coeloglossum viride Var. Bracteatum Extract Attenuates MPTP-Induced Neurotoxicity in vivo by Restoring BDNF-TrkB and FGF2-Akt Signaling Axis and Inhibiting RIP1-Driven Inflammation. FRONT PHARMACOL. 2022 Apr;0:1571 WB ; Mouse. PubMed:35571135 [IF=5.344] Maowen Chen. et al. Osteogenesis regulation of mesenchymal stem cells via autophagy induced by silica–titanium composite surfaces with different mechanical moduli. 2020 Sep 03 IF ; Rat. PubMed:32966545 [IF=5.194] Yu-Shen Luo. et al. Acrylonitrile exposure triggers ovarian inflammation and decreases oocyte quality probably via mitochondrial dysfunction induced apoptosis in mice. CHEM-BIOL INTERACT. 2022 Jun;360:109934 IF ; Mouse. PubMed:35429547 [IF=5.168] Guo-Jian Jiang. et al. Carteolol triggers senescence via activation of β-arrestin–ERK–NOX4–ROS pathway in human corneal endothelial cells in vitro. CHEM-BIOL INTERACT. 2023 Apr;:110511 WB ; Human. PubMed:37120125 [IF=4.872] Shaofeng Wu. et al. Protective effects of curcumin on ATO-induced nephrotoxicity in ducks in relation to suppressed autophagy, apoptosis and dyslipidemia by regulating oxidative stress. Ecotox Environ Safe. 2021 Aug;219:112350 IF ; Duck. PubMed:34022626 [IF=4.85] Yutong Wu. et al. Osteoclast-derived extracellular miR-106a-5p promotes osteogenic differentiation and facilitates bone defect healing. CELL SIGNAL. 2022 Dec;:110549 WB ; Mouse. PubMed:36464103 [IF=4.414] Liu, Zhan. et al. Kaemperfol Protects Dopaminergic Neurons by Promoting mTOR-Mediated Autophagy in Parkinson’s Disease Models. NEUROCHEM RES. 2022 Dec;:1-17 IHC, WB ; Mouse, Rat. PubMed:36469163 [IF=4.285] Xi Huaming. et al. Changes in histology, protein expression, and autophagy in dairy goat testes during non-breeding season. Biol Reprod. 2021 Aug;: IHC ; Goat. PubMed:34467369 [IF=4.109] He Bai. et al. Inhibition of the BNIP3/NIX-dependent mitophagy aggravates copper-induced mitochondrial dysfunction in duck renal tubular epithelial cells. ENVIRON TOXICOL. 2022 Nov;: WB ; Duck. PubMed:36378575 [IF=4.014] Jian Chen. et al. Astragalus polysaccharide alleviates transport stress-induced heart injury in newly hatched chicks via ERS-UPR-Autophagy dependent pathway. POULTRY SCIENCE. 2022 Jun;:102030 WB ; Chicken. PubMed:10.1016/j.psj.2022.102030 [IF=3.776] Qidang Duan. et al. LOX-1 attenuates high glucose-induced autophagy via AMPK/HNF4α signaling in HLSECs. HELIYON. 2022 Dec;8:e12385 WB ; Human. PubMed:36590506 [IF=3.352] Linsong Yang. et al. Influence of Sodium New Houttuyfonate as a New EGFR-TK Inhibitor on the Apoptosis and Autophagy of MCF-7 Cells and Its Toxicity to Caenorhabditis elegans. PROCESSES. 2023 Jun;11(6):1652 ICC ; Human. PubMed:10.3390/pr11061652 [IF=3.246] Shimin Hao. et al. Evaluation of Biodegradable Alloy Fe30Mn0.6N in Rabbit Femur and Cartilage through Detecting Osteogenesis and Autophagy. BIOMED RES INT. 2023 Jan 18;2023:3626776 IHC ; Rabbit. PubMed:36714031 [IF=3.067] Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639. WB&ICF ; Mouse. PubMed:31491480 [IF=3.035] Qian-Qian Hao. et al. Triptolide exposure induces oxidative stress and decreases oocyte quality in mouse. TOXICON. 2023 Jan;221:106964 IF ; Mouse. PubMed:36372154 [IF=2.971] Jiaqi Lu. et al. Andrographolide emeliorates maltol aluminium-induced neurotoxicity via regulating p62-mediated Keap1-Nrf2 pathways in PC12 cells. Pharm Biol. 2021;59(1):232-241 WB ; Rat. PubMed:33632062 [IF=2.967] Shengsen Yang. et al. Effect and mechanism of action of SLP‑2 on the apoptosis and autophagy of gastric cancer cells. Oncol Lett. 2021 Oct;22(4):1-8 WB ; Human. PubMed:34457062 [IF=2.952] Fa-Xiu Chen. et al. Substance P prevents doxorubicin‑induced cardiomyocyte injury by regulating apoptosis and autophagy: In vitro and in vivo evidence. Mol Med Rep. 2022 Feb;25(2):1-9 WB ; Rat. PubMed:34913064 [IF=2.78] Chen, Yunbo, et al. "β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice." Brain research 1552 (2014): 41-54. IHC-P, WB ; Mouse. PubMed:24457043 [IF=2.742] Liu, Yanrong. et al. Cinnamaldehyde affects lipid droplets metabolism after adipogenic differentiation of C2C12 cells. MOL BIOL REP. 2022 Dec;:1-7 WB ; Mouse. PubMed:36538173 [IF=2.535] Chen J et al. Tension induces intervertebral disc degeneration via endoplasmic reticulum stress-mediated autophagy. Biosci Rep. 2019 Jul 8. pii: BSR20190578. WB&IHC ; Rat. PubMed:31285389 [IF=2.323] Xiaorui Fan. et al. Effect of Cryptorchidism on the Histomorphometry, Proliferation, Apoptosis, and Autophagy in Boar Testes. Animals-Basel. 2021 May;11(5):1379 WB,IHC ; Boar. PubMed:34066291 [IF=2.234] Lu et al. Autophagy activator promotes neuronal differentiation of adult adipose-derived stromal cells. (2013) Neural.Regen.Res. 8:882-9 ICC ; Human. PubMed:25206379 [IF=1.713] Jian Wang. et al. Lactobacillus salivarius ameliorated Mycoplasma gallisepticum-induced inflammatory injury and secondary Escherichia coli infection in chickens: Involvement of intestinal microbiota. Vet Immunol Immunop. 2021 Mar;233:110192 WB ; Chicken. PubMed:33476924 [IF=1.56] Li, Min, et al. "Metformin ameliorates hepatic steatosis and improves the induction of autophagy in HFD‑induced obese mice." Molecular Medicine Reports. IHC-P ; Mouse. PubMed:28560428
研究领域	肿瘤细胞生物神经生物学信号转导细胞骨架细胞外基质细胞自噬
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Rat (predicted: Mouse,Chicken,Dog,Pig,Cow,Horse,Sheep,Goat)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	14/16kDa
细胞定位	细胞浆
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human LC3: 31-121/121
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy. Function: Probably involved in formation of autophagosomal vacuoles (autophagosomes). Subunit: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Interacts with SQSTM1. Interacts with TP53INP1 and TP53INP2. Subcellular Location: Cytoplasmic. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3B binds to the autophagic membranes. Tissue Specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. Post-translational modifications: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Similarity: Belongs to the MAP1 LC3 family. SWISS: Q9H492 Gene ID: 84557 Database links: Entrez Gene: 84557 Human Entrez Gene: 66734 Mouse Entrez Gene: 362245 Rat Omim: 601242 Human SwissProt: Q9H492 Human SwissProt: Q91VR7 Mouse SwissProt: Q6XVN8 Rat Unigene: 632273 Human Unigene: 196239 Mouse Unigene: 3135 Rat
产品图片	Protein: mouse brain lysates at 40ug; Primary: rabbit Anti-LC3 (bs-8878R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:14 kD Observed band size:14,16 kD Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3) Polyclonal Antibody, Unconjugated (bs-8878R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3) Polyclonal Antibody, Unconjugated (bs-8878R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): Hela (fixed with 70% ethanol (Overmight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min at -20℃). Primary Antibody (green line): Rabbit Anti-LC3 antibody (bs-8878R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记