| 产品编号 | bs-13530R |
| 英文名称 | GPR31 Rabbit pAb |
| 中文名称 | G蛋白偶联受体31抗体 |
| 别 名 | GPR31_HUMAN; 12-(S)-hydroxy-5, 8, 10, 14-eicosatetraenoic acid receptor; 12-(S)-HETE receptor; 12-HETER; G-protein coupled receptor 31; G protein-coupled receptor 31; GPR31/12-HETER; HETER; HETER1; |
| 研究领域 | 细胞生物 神经生物学 信号转导 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human G protein coupled receptor 31: 121-220/319 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Enables G protein-coupled receptor activity and arachidonic acid binding activity. Involved in G protein-coupled receptor signaling pathway and response to acidic pH. Located in plasma membrane. [provided by Alliance of Genome Resources, Apr 2022] Function: GPCR GPR31 is a member of the G protein coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. GPCR GPR31 is located on human chromosome 6q27. Subcellular Location: Cell membrane; Multi pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: O00270 Gene ID: 2853 Database links: Entrez Gene: 2853 Human Omim: 602043 Human SwissProt: O00270 Human |
| 产品图片 |
Sample: HepG2 (human)Cell Lysate at 40 ug
Primary: Anti-GPR31 (bs-13530R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 36 kD
Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
