产品编号 | bs-13513R |
英文名称 | Rabbit Anti-GPR110 antibody |
中文名称 | G蛋白偶联受体110抗体 |
别 名 | G protein coupled receptor 110; G protein coupled receptor KPG_012; G protein coupled receptor PGR19; G-protein coupled receptor KPG_012; G-protein coupled receptor PGR19; GP110_HUMAN; GPR110; HGPCR36; KPG_012; PGR19; Probable G protein coupled receptor 110; Probable G-protein coupled receptor 110; GPCR GPR110. |
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Specific References (1) | bs-13513R has been referenced in 1 publications.
[IF=4.125] Minghui Zhang. et al. Comparative Transcriptomic Analysis of Mammary Gland Tissues Reveals the Critical Role of GPR110 in Palmitic Acid-Stimulated Milk Protein and Fat Synthesis. BRIT J NUTR. 2023 Mar;:1-32 WB ; Mouse.
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研究领域 | 细胞生物 神经生物学 信号转导 G蛋白偶联受体 G蛋白信号 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse |
产品应用 | WB=1:500-2000,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 99 kDa |
检测分子量 | |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human G protein coupled receptor 110: 51-150/910 <Extracellular> |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR110 (G protein-coupled receptor 110), also known as PGR19, is a 911 amino acid protein that belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. Characterized as an adhesion GPCR, GPR110 is a multipass membrane-bound protein with a long amino-terminus that contains multiple domains. One of these domains is the GPCR proteolytic site (GPS), which is essential for proteolytic cleavage of the amino-terminus and for cell surface expression. Function: Orphan receptor. Subcellular Location: Cell membrane. Similarity: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 1 GPS domain. Contains 1 SEA domain. SWISS: Q5T601 Gene ID: 266977 Database links: Entrez Gene: 266977 Human SwissProt: Q5T601 Human Unigene: 733762 Human |
产品图片 |
Sample: A549 (human)Cell Lysate at 40 ug
Primary: Anti-GPR110(bs-13513R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 109 kD
Sample: A431 (human)Cell Lysate at 40 ug
Primary: Anti-GPR110(bs-13513R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 109 kD
Blank control(blue): Mouse Kidney Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- GPR110 antibody(bs-13513R), Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |