| 产品编号 | bs-13493R |
| 英文名称 | GOSR1 |
| 中文名称 | 高尔基SNAP受体复合物1抗体 |
| 别 名 | 28 kDa cis Golgi SNARE p28; 28 kDa Golgi SNARE protein; Golgi SNAP receptor complex member 1; Golgi SNARE 28 kDa; GOS28; GOS28/P28; GOSR1; GS25; Gs28; P28; GOSR1_HUMAN. |
| 研究领域 | 细胞生物 染色质和核信号 信号转导 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 29kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GOSR1/GS28: 151-250/250 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
In eukaryotic cells, the Golgi apparatus receives newly synthesized proteins from the endoplasmic reticulum and delivers them after covalent modification to their destination in the cell. For membrane-directed proteins this process is believed to be carried out via vesicular transport. Correct vesicular transport is determined by specific pairing of vesicle-associated SNAREs (v-SNAREs) with those on the target membrane (t-SNAREs). This complex then recruits soluble NSF attachment proteins (SNAPs) and N-ethylmaleimide-sensitive factor (NSF) to form the highly stable SNAP receptor (SNARE) complex. The formation of a SNARE complex pulls the vesicle and target membranes together and may provide the energy to drive the fusion of the lipid bilayers. Golgi SNARE 27 kDa (GS27) and GS28 belong to the SNARE protein family and are important trafficking proteins between the endoplasmic reticulum and the Golgi and between Golgi subcompartments. GS27 and GS28 both exist as cytoplasmically oriented integral membrane proteins. The human GS27 gene, which maps to chromosome 17q21, is located near a locus implicated in familial essential hypertension, indicating that it is a potential candidate gene for this disease. The human GS28 gene maps to chromosome 17q11. Function: GOSR1 is a trafficking membrane protein which transports proteins among the endoplasmic reticulum and the Golgi and between Golgi compartments; it is considered an essential component of the Golgi SNAP receptor (SNARE) complex. Subunit: Component of several multiprotein Golgi SNARE complexes. Identified in a SNARE complex with BET1, STX5 and YKT6, in a SNARE complex with BET1L, STX5 and YKT6, in a SNARE complex with STX5, GOSR2, SEC22B and BET1, and in complex with STX5 and COG3. Interacts with GABARAPL2 (By similarity). Subcellular Location: Cell Membrane, Endoplasmic reticulum and Golgi Apparatus Similarity: Belongs to the GOSR1 family. SWISS: O95249 Gene ID: 9527 Database links: Entrez Gene: 9527 Human Entrez Gene: 53334 Mouse Omim: 604026 Human SwissProt: O95249 Human SwissProt: O88630 Mouse Unigene: 462680 Human Unigene: 20931 Mouse Unigene: 8228 Rat |
| 产品图片 |
Sample:
NIH/3T3 Cell Lysate at 40 ug Primary: Anti-GOSR1 (bs-13493R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD 
Sample:
Embryo (Mouse) Lysate at 40 ug Primary: Anti-GOSR1 (bs-13493R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD 
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GOSR1) Polyclonal Antibody, Unconjugated (bs-13493R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
