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Rabbit Anti-phospho-CD130/gp130 (Ser782)  antibody (bs-10122R)  
~~~促销代码KT202411~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-10122R
英文名称 Rabbit Anti-phospho-CD130/gp130 (Ser782)  antibody
中文名称 磷酸化gp130抗体
别    名 gp130 (phospho S782); gp130 (phospho Ser782); p-gp130 (Ser782); Interleukin-6 receptor subunit beta; IL-6R-beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; CDw130; Oncostatin-M receptor subunit alpha; CD_antigen; CD130; GP130 RAPS; gp130 transducer chain; GP130-RAPS; IL6 ST; IL6R-beta; IL6ST.IL6R-beta; IL6RB_HUMAN; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB.  
产品类型 磷酸化抗体 
研究领域 细胞生物  信号转导  细胞膜受体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Rat,Rabbit (predicted: Mouse,Pig,Cow,Chicken,Dog,Horse)
产品应用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
细胞定位 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human gp130 around the phosphorylation site of Ser782: SE(p-S)TQ 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Function:
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.

Subunit:
Interacts with INPP5D/SHIP1. Forms heterodimers composed of LIPR and IL6ST (type I OSM receptor). Also forms heterodimers composed of OSMR and IL6ST (type II OSM receptor). Homodimer. The homodimer binds two molecules of herpes virus IL6. Component of a hexamer of two molecules each of IL6, IL6R and IL6ST. Interacts with HCK.

Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Tissue Specificity:
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.

Post-translational modifications:
Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated.

Similarity:
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

SWISS:
P40189

Gene ID:
3572

Database links:

Entrez Gene: 3572 Human

Entrez Gene: 16195 Mouse

Entrez Gene: 25205 Rat

Omim: 600694 Human

SwissProt: P40189 Human

SwissProt: Q00560 Mouse

SwissProt: P40190 Rat

Unigene: 532082 Human

Unigene: 706627 Human

Unigene: 4364 Mouse

Unigene: 12138 Rat



产品图片
Sample: Lane 1: Human HepG2 cell Lysates Lane 2: Human HUVEC cell Lysates Primary: Anti-phospho-CD130gp130 (Ser782) (bs-10122R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130kDa Observed band size: 130kDa
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (bs-10122R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (bs-10122R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
phosphopeptide non phosphopeptide
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