| 产品编号 | bs-10170R |
| 英文名称 | FLT3L |
| 中文名称 | FMS样酪氨酸激酶3配体抗体 |
| 别 名 | Flt 3 ligand; Flt 3L; Flt3 L; FLT3 LG; Flt3 ligand; Flt3L; FLT3L_HUMAN; FLT3LG; Fms related tyrosine kinase 3 ligand; Fms-related tyrosine kinase 3 ligand; SL cytokine. |
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Specific References (1) | bs-10170R has been referenced in 1 publications.
[IF=3.816] Lun Yao. et al. Recombinant Pseudorabies Virus with TK/gE Gene Deletion and Flt3L Co-Expression Enhances the Innate and Adaptive Immune Response via Activating Dendritic Cells. Viruses-Basel. 2021 Apr;13(4):691 WB,IF ; Pig.
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| 研究领域 | 心血管 细胞生物 干细胞 细胞分化 淋巴细胞 t-淋巴细胞 b-淋巴细胞 细胞因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Human (predicted: Horse,Cow,Pig,Dog,Rat) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23kDa |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FLT3L: 61-160/235 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Flt 3 ligand (Flt 3-L), variously designated Flt 3/Flk 2 ligand or FL, is a hematopoietic growth factor that stimulates the proliferation of stem and CD34+ progenitor cells and has been cloned from both mouse and human genomes. Flt 3-L is a potent in vitro growth stimulator of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and G-CSF-dependent granulocyte-macrophage committed precursors from Lin CD34+ bone marrow cells as well as other primitive B cell populations. Additionally, Flt 3-L stimulates the proliferation of hematopoietic progenitor cells isolated from mouse fetal liver or adult mouse bone marrow. Flt 3-L does not, however, affect the growth of erythroid-committed progenitors. Flt 3-L exists in two forms and is active as both a soluble and as a membrane-bound ligand. The Flt 3-L receptor, Flt 3, is a tyrosine kinase expressed on CD34+ cells that shares a high degree of homology with the SCF (stem cell factor) receptor, c-Kit and c-Fms. Function: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. Subunit: Homodimer (isoform 2). Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. SWISS: P49771 Gene ID: 2323 Database links: Entrez Gene: 2323 Human Entrez Gene: 719239 Rhesus monkey Omim: 600007 Human SwissProt: P49771 Human Unigene: 428 Human
Flt3L是一种可以促进多种干细胞、血细胞及其前体细胞生成、分化的细胞因子,对T淋巴细胞、B淋巴细胞和NK细胞和DC的前体细胞都有促进增生、诱导分化的功能.Flt3L可与其它促细胞生长的细胞因子有很好的协同作用.目前,尚未发现具有毒性作用,是一种良好的扩增剂,对研究细胞的形态、细胞表现型和功能具有重要的意义. |
| 产品图片 |
Sample: intestine (Mouse) Lysate at 40 ug
Primary: Anti-FLT3L(bs-10196R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 28 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
