| 产品编号 | bs-13197R |
| 英文名称 | FOPNL Rabbit pAb |
| 中文名称 | 胚胎干细胞相关蛋白FOPNL抗体 |
| 别 名 | C16orf63; FGFR1OP N terminal like; FGFR1OP N-terminal-like protein; FOP-related protein of 20 kDa; Fopnl; FOPNL_HUMAN; FOR20; LisH domain containing protein C16orf63; LisH domain-containing protein FOPNL; PHSECRG2; Pluripotent embryonic stem cell related protein. |
| 研究领域 | 肿瘤 免疫学 发育生物学 神经生物学 干细胞 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 20 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FOPNL/C16orf63: 6-80/174 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
C16orf63, also known as FLJ31153 or DKFZp686N1651, is a 174 amino acid protein that contains one LisH domain. The gene that encodes C16orf63 maps to human chromosome 16. Chromosome 16 encodes over 900 genes in approximately 90 million base pairs, makes up nearly 3% of human cellular DNA and is associated with a variety of genetic disorders. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, though through the CREBBP gene which encodes a critical CREB binding protein. Signs of Rubinstein-Taybi include mental retardation and predisposition to tumor growth and white blood cell neoplasias. Crohn's disease is a gastrointestinal inflammatory condition associated with chromosome 16 through the NOD2 gene. An association with systemic lupus erythematosis and a number of other autoimmune disorders with the pericentromeric region of chromosome 16 has led to the identification of SLC5A11 as a potential autoimmune modifier. The C16orf63 gene product has been provisionally designated C16orf63 pending further characterization. Function: Involved in the biogenesis of cilia. Subunit: Homooligomer; probably required for localization to centrosomes. Subcellular Location: Cell projection; cilium. Cytoplasm; cytoskeleton; cilium basal body. Cytoplasm; cytoskeleton; centrosome. Cytoplasmic granule. Localizes to pericentriolar satellites, small (70-100 nm) cytoplasmic granules associated with the centrosome. Tissue Specificity: Widely expressed. Detected in brain, heart, kidney, liver, lung, skeletal muscle, placenta and intestine. Similarity: Belongs to the FGFR1OP family. Contains 1 LisH domain. SWISS: Q96NB1 Gene ID: 123811 Database links: Entrez Gene: 123811 Human SwissProt: Q96NB1 Human Unigene: 514179 Human |
| 产品图片 |
Sample:
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti- FOPNL (bs-13197R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 19 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Anti
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
