| 产品编号 | bs-0220P |
| 英文名称 | Alkaline Phosphatase(≥5,500 U/mg) |
| 中文名称 | 碱性磷酸酶 (进口分装 ≥5,500 U/mg)蛋白 |
| 别 名 | Alkaline Phosphatase from calf intestine; Phosphatase, Alkaline from bovine intestinal mucosa; Alkaline phosphatase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); ALP-1; ALP; ALPP; ALPPL2; ALPPL; PALP; PLAP-1; PLAP; |
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Specific References (1) | bs-0220P has been referenced in 1 publications.
[IF=3.864] Xie W et al. A high-resolution colorimetric immunoassay platform realized by coupling enzymatic multicolor generation with smartphone readout.Analyst. 2018 Jun 11;143(12):2901-2907. other ;
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| 保存条件 | Stored at 2-8℃. Do not freeze. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Alkaline phosphatase is highly purified from calf intestine. Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. Typical uses in the lab for alkaline phosphatases include removing phosphate monoesters to prevent self-ligation, which is undesirable during plasmid DNA cloning. Another important use of alkaline phosphatase is as a label for enzyme immunoassays. SWISS: N/A Gene ID: N/A CAS：9001-78-9 分子量：dimer ~160 kDa MDL：MFCD00131849 浓度：≥10 mg/mL 活性：≥5500 DEA单位/mg蛋白质 单位定义：一个DEA单位将在37°C的pH 9.8下每分钟水解1μmol 4-硝基苯磷酸酯（一个甘氨酸单位相当于~3个DEA单位） 性状：溶液（含5 mM Tris、5 mM MgCl2和0.1 mM ZnCl2的50%甘油溶液，pH 7.0） 用途：碱性磷酸酶可以用来去磷酸酪蛋白和其他蛋白质。碱性磷酸酶也可以用来去磷酸化DNA或RNA的5′端，以防止自连接。用碱性磷酸酶去磷酸化后，DNA或RNA也可以用放射性标记的磷酸盐（通过T4多核苷酸激酶）标记。 推荐用于抗体和蛋白质结合的高比活性等级。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
