| 产品编号 | bs-13169R |
| 英文名称 | FENS1 |
| 中文名称 | 磷酸肌醇结合蛋白1抗体 |
| 别 名 | FENS 1; FENS-1; KIAA1435; Phosphoinositide binding protein 1; Phosphoinositide binding protein SR1; Phosphoinositide-binding protein 1; WD repeat and FYVE domain containing 1; WD repeat and FYVE domain-containing protein 1; WD40 and FYVE domain containing protein 1; WD40- and FYVE domain-containing protein 1; WDF1; WDFY1; WDFY1_HUMAN; ZFYVE17; Zinc finger FYVE domain containing protein 17; Zinc finger FYVE domain-containing protein 17. |
 |
Specific References (2) | bs-13169R has been referenced in 2 publications.
[IF=7.561] Alexandru Tatomir. et al. RGC-32 Acts as a Hub to Regulate the Transcriptomic Changes Associated With Astrocyte Development and Reactive Astrocytosis. Front Immunol. 2021; 12: 705308 IHC ; Mouse.
[IF=3.921] Anna Sancho-Balsells et al. Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1. Front Cell Neurosci. 2020 May 14;14:93. WB ; Mouse.
|
| 研究领域 | 细胞生物 信号转导 转运蛋白 结合蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Pig,Cow,Horse,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FENS1: 21-120/410 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. WDFY1 (WD repeat and FYVE domain containing 1), also known as WDF1, FENS-1 or ZFYVE17, is a 410 amino acid protein that localizes to the early endosome and contains one FYVE-type zinc finger and seven WD repeats through which it may play a role in protein trafficking and signal transduction. Subunit: Binds PtdIns3P in vitro with high specificity over other phosphoinositides. Subcellular Location: Early endosome. Similarity: Contains 1 FYVE-type zinc finger. Contains 7 WD repeats. SWISS: Q8IWB7 Gene ID: 57590 Database links: Entrez Gene: 57590 Human Entrez Gene: 69368 Mouse SwissProt: Q8IWB7 Human Unigene: 368359 Human Unigene: 8557 Rat |
| 产品图片 |
Sample:
Lane 1: Cerebrum (Mouse) Tissue Lysate at 40 ug Lane 2: Cerebrum (Rat) Tissue Lysate at 40 ug Lane 3: Skin (Rat) Tissue Lysate at 40 ug Lane 4: Huvec (Human) Cell Lysate at 30 ug Lane 5: HepG2 (Human) Cell Lysate at 30 ug Lane 6: THP-1 (Human) Cell Lysate at 30 ug Primary: Anti-FENS1 (bs-13169R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 48 kD 
Sample:
Lane 1: U87MG (Human) Cell Lysate at 30 ug Lane 2: HepG2 (Human) Cell Lysate at 30 ug Lane 3: U2os (Human) Cell Lysate at 30 ug Lane 4: A431 (Human) Cell Lysate at 30 ug Primary: Anti-FENS1 (bs-13169R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD 
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FENS1) Polyclonal Antibody, Unconjugated (bs-13169R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
