| 产品编号 | bs-13166R |
| 英文名称 | FbxL4 |
| 中文名称 | F-box富含亮氨酸重复蛋白4抗体 |
| 别 名 | F box and leucine rich repeat protein 4; F box protein FBL4/FBL5; F box/LRR repeat protein 4; F-box and leucine-rich repeat protein 4; F-box protein FBL 4; F-box protein FBL4/FBL5; F-box/LRR-repeat protein 4; FBL 4; FBL 5; FBL4; FBL5; Fbxl4; FBXL4_HUMAN. |
| 研究领域 | 细胞生物 细胞周期蛋白 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Chicken,Cow,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 70kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FbxL4/FBL4: 15-100/621 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least 9 tandem leucine-rich repeats. [provided by RefSeq, Jul 2008]. Subunit: Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity). Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Expressed in heart, kidney, liver, lung, pancreas, and placenta, but not in skeletal muscle. Similarity: Contains 1 F-box domain. Contains 9 LRR (leucine-rich) repeats. SWISS: Q9UKA2 Gene ID: 26235 Database links: Entrez Gene: 26235 Human Entrez Gene: 269514 Mouse Omim: 605654 Human SwissProt: Q9UKA2 Human SwissProt: Q8BH70 Mouse Unigene: 536850 Human Unigene: 40708 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FbxL4) Polyclonal Antibody, Unconjugated (bs-13166R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FbxL4) Polyclonal Antibody, Unconjugated (bs-13166R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
