| 产品编号 | bs-13160R |
| 英文名称 | Fibulin 2 |
| 中文名称 | 纤连蛋白2抗体 |
| 别 名 | Fibulin-2; Fbln2; FBLN2_HUMAN; FIBL-2; Fibulin-2; OTTHUMP00000216670; OTTHUMP00000216671; OTTHUMP00000216672. |
| 研究领域 | 肿瘤 细胞生物 神经生物学 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Cow,Sheep) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 124kDa |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Fibulin 2: 501-600/1184 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Fibulin-1 and Fibulin-2 associate with Fibronectin and other extracellular matrix proteins. In bone marrow, Fibulin-1 and Fibulin-2 bind to Fibronectin in the adherent layer. Fibulin-1 expression is stimulated by estrogen in ovarian cancer cell lines and has been suggested as both an agent of metastasis in ovarian cancer cells and as an indicator for predicting cancer risk or aggressiveness in ovarian carcinomas. The mobility of cancer cells may be inhibited with increasing exposure to Fibulin-1. Fibulin-2 binds to the lectin domains of extracellular matrix proteins aggrecan, versican and brevican. Fibulin-2 is abundantly expressed in heart, placenta and ovarian tissue, where it localizes to basement membranes and connective tissue compartments. In mice, differential Fibulin-2 gene expression correlates with the early phase of diabetic kidneys and glomerulosclerosis. The gene encoding human Fibulin-2 maps to chromosome 3p25.1. Function: Its binding to fibronectin and some other ligands is calcium dependent. Subunit: Homotrimer; disulfide-linked. Interacts with LAMA2 (By similarity). Subcellular Location: Secreted; extracellular space; extracellular matrix. Tissue Specificity: Component of both basement membranes and other connective tissues. Expressed in heart, placenta and ovary. Post-translational modifications: O-glycosylated with core 1 or possibly core 8 glycans. It is unsure if the O-glycosylation is on Thr-347 or Ser-348. Similarity: Belongs to the fibulin family. Contains 3 anaphylatoxin-like domains. Contains 11 EGF-like domains. SWISS: P98095 Gene ID: 2199 Database links: Entrez Gene: 2199 Human Entrez Gene: 14115 Mouse Omim: 135821 Human SwissProt: P98095 Human SwissProt: P37889 Mouse Unigene: 198862 Human Unigene: 249146 Mouse Unigene: 410235 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
