| 产品编号 | bs-13144R |
| 英文名称 | FARP1 |
| 中文名称 | 软骨细胞Ezrin样蛋白抗体 |
| 别 名 | CDEP; Chondrocyte derived ezrin like protein; FERM RhoGEF and pleckstrin domain containing protein 1; FERM RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived). PLEKHC2; FARP1_HUMAN. |
| 研究领域 | 细胞生物 神经生物学 细胞类型标志物 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 119kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FARP1: 201-300/1045 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FARP1 (FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1), also known as PLEKHC2 or CDEP, is a 1,045 amino acid protein that contains one FERM domain, one DH domain and two PH domains. Existing as multiple alternatively spliced isoforms that are expressed in fetal heart, brain and spleen, as well as in adult lung, kidney and testis, FARP1 is thought to function as a Rho-guanine nucleotide exchange factor that may play a role in linking the cell membrane to the cytoskeleton. The gene encoding FARP1 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections. Function: The FARP1 protein contains a predicted ezrin-like domain, a Dbl homology domain, and a pleckstrin homology domain, and is believed to be a member of the band 4.1 superfamily, whose members link the cytoskeleton to the cell membrane. Two alternatively spliced transcript variants encoding distinct isoforms have been found for the FARP1 gene, which shows increased expression in differentiated chondrocytes versus dedifferentiated chondrocytes. Subcellular Location: Cytoplasmic. Similarity: Contains 1 DH (DBL-homology) domain. Contains 1 FERM domain. Contains 2 PH domains. SWISS: Q9Y4F1 Gene ID: 10160 Database links: Entrez Gene: 10160 Human Omim: 602654 Human SwissProt: Q9Y4F1 Human Unigene: 403917 Human |
| 产品图片 |
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-FARP1 antibody (bs-13144R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
