| 产品编号 | bs-13130R |
| 英文名称 | Factor D Rabbit pAb |
| 中文名称 | 补体因子D抗体 |
| 别 名 | ADIPSIN; ADN; DF; PFD; CFAD_HUMAN; CFD; C3 convertase activator; Properdin factor D; 3.4.21.46; |
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Specific References (2) | bs-13130R has been referenced in 2 publications.
[IF=5.606] Miyagawa Takuya. et al. Fli1 deficiency induces endothelial adipsin expression, contributing to the onset of pulmonary arterial hypertension in systemic sclerosis. Rheumatology. 2020 Aug;59(8):2005-2015 IHC ; Human.
[IF=4.357] Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137 WB ; Human.
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 24 kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Factor D/Adipsin: 21-120/253 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Adipsin is the mouse homolog of the previously described human complement Factor D, a serine protease, which is now designated human Adipsin. Human Adipsin is highly expressed in and secreted by adipose tissue, and it has also been found in monocytes and macrophages. Rodent Adipsin has only been detected in high levels in adipose tissue. It has been shown that complement factor B, when complexed with activated complement component C3, is cleaved by Adipsin. While low expression of Adipsin has been confirmed in obese mice with hypothalamic defects, this inverse correlation between Adipsin expression and obesity has not been demonstrated in humans. Function: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Subcellular Location: Secreted. DISEASE: Defects in CFD are the cause of complement factor D deficiency (CFD deficiency) [MIM:134350]. CFD deficiency predisposes to invasive meningococcal disease. Similarity: Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. SWISS: P00746 Gene ID: 1675 Database links: Entrez Gene: 1675 Human Omim: 134350 Human SwissProt: P00746 Human Unigene: 155597 Human |
| 产品图片 |
Protein:
A549(human)cell lysates at 40ug;
spleen disease in mouse at 40ug ;
Primary: Anti-Factor D (bs-13130R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size :24 kD
Observed band size :28/75 kD
Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Factor D Polyclonal Antibody, Unconjugated(bs-13130R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
