| 产品编号 | bs-10023R |
| 英文名称 | STAT4 |
| 中文名称 | 信号转导和转录激活因子4抗体 |
| 别 名 | signal transducers and activators of transduction 4; CIS4; HSPC060; SOCS4; SOCS6; SSI4; STAI4; STAT 4; STAT4; STATI4; Suppressor of cytokine signaling 6; STAT4_HUMAN. |
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Specific References (3) | bs-10023R has been referenced in 3 publications.
[IF=5.714] Chenglin Li. et al. Ranitidine as an adjuvant regulates macrophage polarization and activates CTLs through the PI3K-Akt2 signaling pathway. INT IMMUNOPHARMACOL. 2023 Mar;116:109729 WB ; Mouse.
[IF=3.943] Le Tian. et al. Luteolin as an adjuvant effectively enhances CTL anti-tumor response in B16F10 mouse model. Int Immunopharmacol. 2021 May;94:107441 WB ; Mouse.
[IF=3.487] Ran Lu. et al. Chrysin Enhances Anti-tumor Immunity Response through IL-12-STAT4 Signal Pathway in B16F10 Melanoma Mouse Model. SCAND J IMMUNOL. 2022 Apr 28 WB ; Mouse.
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| 研究领域 | 肿瘤 细胞生物 发育生物学 信号转导 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Dog,Pig,Cow,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 85kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human STAT4: 111-210/748 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]. Function: Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling. Subunit: Forms a homodimer or a heterodimer with a related family member. Interacts with ARL2BP. The SH2 domain interacts, in vitro, with IL12RB2 via a short cytoplasmic domain. Subcellular Location: Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation. Post-translational modifications: Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity. DISEASE: Genetic variations in STAT4 are associated with susceptibility to systemic lupus erythematosus type 11 (SLEB11) [MIM:612253]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Genetic variations in STAT4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Similarity: Belongs to the transcription factor STAT family. Contains 1 SH2 domain. SWISS: Q14765 Gene ID: 6775 Database links: Entrez Gene: 6775 Human Entrez Gene: 20849 Mouse Omim: 600558 Human SwissProt: Q14765 Human SwissProt: P42228 Mouse Unigene: 735572 Human Unigene: 80642 Human Unigene: 1550 Mouse Unigene: 137580 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
