| 产品编号 | bs-13102R |
| 英文名称 | Epsin 2 Rabbit pAb |
| 中文名称 | 内吞作用辅助蛋白EPN2抗体 |
| 别 名 | EHB21; EPN 2; EPN-2; Epn2; EPN2_HUMAN; EPS 15 interacting protein 2; EPS-15-interacting protein 2; Eps15 binding protein; Epsin 2; Epsin-2; Epsin2; KIAA1065; OTTHUMP00000065808; OTTHUMP00000065809; OTTHUMP00000065810; OTTHUMP00000065811; OTTHUMP00000065889. |
| 研究领域 | 肿瘤 细胞生物 生长因子和激素 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Epsin 2: 31-130/641 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation. Miscues in RTK signaling pathways can result in cellular transformation and ultimately in cancer. Two novel EGF receptor substrates have been described, designated EGF-receptor pathway substrates 8 and 15, or Eps8 and Eps15. Epsin is a binding partner to Eps15. Both epsin and Eps15 have an ubiquitous tissue distribution but are concentrated in presynaptic nerve terminals specialized for the Clathrin-mediated endocytosis of synaptic vesicles. Disruption of epsin function blocks Clathrin-mediated endocytosis. Epsin, along with its binding partner Eps15, is proposed to be involved in the assistance of Clathrin coat rearrangement during Clathrin coated pit invagination. Epsin 2a, and 2b are splicing variants of epsin 2, which is associated with Clathrin-mediated endocytosis and are enriched in the brain in the peri-Golgi region. Function: Plays a role in the formation of clathrin-coated invaginations and endocytosis. Subunit: Binds EPS15 (By similarity). Binds AP-2 and clathrin. Subcellular Location: Cytoplasm. Cytoplasmic vesicle; clathrin-coated vesicle. In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex. Tissue Specificity: Highest expression is found in brain. Detected at lower levels in lung and liver. Post-translational modifications: Ubiquitinated. Similarity: Belongs to the epsin family. Contains 1 ENTH (epsin N-terminal homology) domain. Contains 2 UIM (ubiquitin-interacting motif) repeats. SWISS: O95208 Gene ID: 22905 Database links: Entrez Gene: 22905 Human Entrez Gene: 13855 Mouse Omim: 607263 Human SwissProt: O95208 Human SwissProt: Q8CHU3 Mouse Unigene: 309467 Human Unigene: 139695 Mouse Unigene: 436466 Mouse Unigene: 44273 Rat |
| 产品图片 |
Sample: HepG2(human) Cell Lysate at 40 ug
Primary: Anti-Epsin2(bs-13102R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Epsin 2) Polyclonal Antibody, Unconjugated (bs-13102R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
