| 产品编号 | bs-13064R |
| 英文名称 | FABP12 |
| 中文名称 | 脂肪酸结合蛋白12抗体 |
| 别 名 | FABP12; Fatty acid binding protein ORF; Fatty acid-binding protein 12; FBP12_HUMAN; Intracellular fatty acid binding protein FABP12; Probable fatty acid binding protein ENSP00000353650. |
| 研究领域 | 细胞生物 信号转导 结合蛋白 新陈代谢 跨膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Dog,Cow,Horse,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 16kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FABP12: 21-120/140 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Fatty acid-binding proteins, designated FABPs, are a family of homologous cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABP12 (fatty acid-binding protein 12) is a 132 amino acid protein that belongs to the calycin superfamily and fatty-acid binding protein family. Highly expressed in adult retina and testis, FABP12 may function in lipid transport. The gene encoding FABP12 maps to mouse chromosome 3 A1. Function: May play a role in lipid transport. Tissue Specificity: Expressed in a number of retinoblastoma cell lines. Similarity: Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. SWISS: A6NFH5 Gene ID: 646486 Database links: Entrez Gene: 646486 Human Entrez Gene: 75497 Mouse SwissProt: A6NFH5 Human SwissProt: Q9DAK4 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FABP12) Polyclonal Antibody, Unconjugated (bs-13064R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FABP12) Polyclonal Antibody, Unconjugated (bs-13064R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
