| 产品编号 | bs-13052R |
| 英文名称 | Rabbit Anti-UBR5 antibody |
| 中文名称 | E3泛素蛋白连接酶EDD抗体 |
| 别 名 | 4432411E13Rik; AW549941; C77315; D030042K14; DD5; E3 identified by differential display; E3 ubiquitin protein ligase, HECT domain containing, 1; E3 ubiquitin-protein ligase; E3 ubiquitin-protein ligase UBR5; EDD 1; EDD; EDD1; FLJ11310; HECT domain-containing 1; hHYD; HYD; Hyperplastic discs protein homolog; KIAA0896; MGC57263; mKIAA0896; Progestin induced protein; Progestin-induced protein; Rat100; Ubiquitin protein ligase; Ubiquitin protein ligase E3 component n recognin 5; UBR5; UBR5_HUMAN. |
| 研究领域 | 细胞生物 表观遗传学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Pig,Sheep,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 309kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EDD: 11-120/2799 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
EDD is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth. EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase. Function: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Subunit: Binds TOPBP1. Associates with CDK9 and TFIIS/TCEA1 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulate target gene transcription (e.g. gamma fibrinogen/FGG) by recruiting their promoters. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts directly with DYRK2. Subcellular Location: Nucleus. Tissue Specificity: Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 1 PABC domain. Contains 1 UBR-type zinc finger. SWISS: O95071 Gene ID: 51366 Database links: Entrez Gene: 51366 Human Entrez Gene: 70790 Mouse Omim: 608413 Human SwissProt: O95071 Human SwissProt: Q80TP3 Mouse Unigene: 492445 Human Unigene: 476840 Mouse Unigene: 54812 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBR5) Polyclonal Antibody, Unconjugated (bs-13052R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
