| 产品编号 | bs-13025R |
| 英文名称 | Dnmt2 Rabbit pAb |
| 中文名称 | DNA甲基转移酶2抗体 |
| 别 名 | dDNMT; DmMT 2; DmMT2; DNA(cytosine 5 ) methyltransferase 2; DNA(cytosine 5) methyltransferase like protein 2; DNA(cytosine-5)-methyltransferase-like protein 2; DNA 5 cytosine methyltransferase; DNA methyltransferase 2; DNA methyltransferase homolog HsaIIP; DNA MTase homolog HsaIIP; Dnmt 2; Dnmt2; M.HsaIIP; MHsaIIP; nmt 2; nmt2; OTTHUMP00000045198; PuMet; RNMT 1; RNMT1; TRDMT 1; TRDMT_HUMAN; TRDMT1; tRNA(cytosine 5 ) methyltransferase; tRNA(cytosine(38)-C(5))-methyltransferase; tRNA aspartic acid methyltransferase 1; tRNA aspartic acid methyltransferase 1 variant 1; tRNA aspartic acid methyltransferase 1 variant 2; tRNA aspartic acid methyltransferase 1 variant 3; tRNA aspartic acid methyltransferase 1 variant 4; tRNA aspartic acid methyltransferase 1 variant 5; tRNA aspartic acid methyltransferase 1 variant 8. |
| 研究领域 | 细胞生物 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human,Mouse,Rat,Pig,Sheep,Cow) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 45 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Dnmt2: 61-160/391 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA. Function: Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes. Similarity: Belongs to the C5-methyltransferase family. SWISS: O14717 Gene ID: 1787 Database links:
Entrez Gene: 1787 Human SwissProt: O14717 human 取消WB,姬2018.7.19； |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
