| 产品编号 | bs-15124R |
| 英文名称 | Rabbit Anti-C21orf33 antibody |
| 中文名称 | 21号染色体开放阅读框33抗体 |
| 别 名 | Chromosome 21 open reading frame 33; D21S2048E; ES 1; ES1; Es1 protein; ES1 protein homolog mitochondrial; GT335; HES 1; HES1; Human HES1 protein homolog to E.coli and zebrafish ES1 protein; Keio novel protein I; KNP I; KNPH; KNPI; Protein GT335; Protein KNP I; ES1_HUMAN; GATD3A |
| 研究领域 | 细胞生物 免疫学 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Chicken,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human C21orf33: 101-200/268 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
KNP-I is a ubiquitously expressed mitochondrial protein that belongs to the ES1 family of proteins. It is a highly conserved protein with homologs identified in various species. This conserved nature suggests an important biological role for the KNP-I protein. The gene that encodes KNP-I (C21orf33) is located on human chromosome 21 in close proximity to a region (D21S25) associated with many genetic diseases. Down syndrome (DS), caused by an extra copy of chromosome 21, is the most common manifestation of trisomic chromosomes. It is likely that the overproduction of the C21orf33 gene product plays a role in the pathology of DS, while its chromosomal location suggests its likely involvement in D21S25 related diseases. While expressed in most tissue, highest expression of KNP-I is found in heart and muscle Subcellular Location: Mitochondrion (Potential). Tissue Specificity: Ubiquitous, but strongly expressed in heartand skeletal muscle. Similarity: Belongs to the ES1 family. Database links: Entrez Gene: 8209 Human Omim: 601659 Human SwissProt: P30042 Human Unigene: 413482 Human |
| 产品图片 |
Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-C21orf33 (bs-15124R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Tissue/cell: Rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-C21orf33 Polyclonal Antibody, Unconjugated(bs-15124R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1ug in 100 uL1X PBS containing 0.5% BSA(green).
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
