| 产品编号 | bs-12952R |
| 英文名称 | phospho-Acetyl Coenzyme A Carboxylase beta (Ser220) |
| 中文名称 | 磷酸化乙酰辅酶A羧化酶抗体 |
| 别 名 | Acetyl Coenzyme A Carboxylase beta (phospho S221); Acetyl Coenzyme A Carboxylase beta (phospho Ser221); p-Acetyl Coenzyme A Carboxylase beta (phospho S221); Acetyl Coenzyme A Carboxylase; ACAS2; AceCS; Acetate CoA ligase; Acetyl CoA synthetase; Acetyl coenzyme A synthetase cytoplasmic; Acetyl-CoA synthetase; ACS; ACSS2; Acyl activating enzyme; Acyl CoA synthetase short chain family member 2; EC 6.2.1.1; MYH7B; ACACB; ACC beta; ACC2; ACCB; Acetyl CoA carboxylase 2; Acetyl CoA carboxylase beta; Biotin carboxylase; COA2; HFA1; ACACB_HUMAN. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 心血管 细胞生物 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 265kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human Acetyl Coenzyme A Carboxylase beta around the phosphorylation site of Ser220: RP(p-S)M |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Function: ACC-beta may be involved in the provision of malonyl-CoA or in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. Subunit: Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity. Subcellular Location: Endomembrane system. Note=May associate with membranes. Tissue Specificity: Predominantly expressed in the heart, skeletal muscles and liver. Similarity: Contains 1 ATP-grasp domain. Contains 1 biotin carboxylation domain. Contains 1 biotinyl-binding domain. Contains 1 carboxyltransferase domain. SWISS: O00763 Gene ID: 32 Database links: Entrez Gene: 31 Human Entrez Gene: 32 Human Entrez Gene: 100705 Mouse Entrez Gene: 107476 Mouse Omim: 200350 Human Omim: 601557 Human SwissProt: O00763 Human SwissProt: Q13085 Human SwissProt: Q5SWU9 Mouse Unigene: 160556 Human Unigene: 234898 Human Unigene: 44372 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
