| 产品编号 | bs-12880R |
| 英文名称 | BOULE |
| 中文名称 | 精子成熟相关蛋白BOULE抗体 |
| 别 名 | Bol (Drosophila boule homolog) like; Bol; Bol boule like; Bol, boule like (Drosophila); bol, boule-like (Drosophila); Boll; BOLL_HUMAN; BOULE; Boule like; BOULE, Drosophila, homolog of; Protein boule like; Protein boule-like; Putative uncharacterized protein BOLL. |
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Specific References (1) | bs-12880R has been referenced in 1 publications.
[IF=1.832] Li T et al. Histomorphological Comparisons and Expression Patterns of BOLL Gene in Sheep Testes at Different Development Stages.Animals (Basel). 2019 Mar 21;9(3). WB&IHC ; Sheep.
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| 研究领域 | 细胞生物 发育生物学 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Dog,Pig,Cow,Sheep,Monkey) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BOULE: 1-100/283 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Spermatogenesis represents the intricate developmental process of mitotic and meiotic cell divisions that ultimately leads to the production of haploid spermatozoa. BOULE, a member of the human deleted in azoospermia (DAZ) family, functions as a key conserved switch that regulates the progression of germ cells through meiosis in man. BOULE is an RNA-binding protein that regulates the expression of twine, a Cdc25 phosphatase, which promotes progression through meiosis. BOULE is expressed not only in the testis, but also in the nervous system, where it may play a role in neural communication. Mutations in the BOULE gene are be associated with male infertility, and the relative proportions of the three BOULE isoforms (B1, B2 and B3) may function as predictive markers for meiotic efficiency. Function: Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation. Subunit: Interacts with DAZ1 and DAZL. Subcellular Location: Cytoplasm. Tissue Specificity: Testis specific. Not expressed in early embryos, primordial germ cells and spermatogonial cells. First expressed in the cytoplasm of spermatocytes and then persists through meiosis. Similarity: Belongs to the RRM DAZ family. Contains 1 DAZ-like domain. Contains 1 RRM (RNA recognition motif) domain. SWISS: Q8N9W6 Gene ID: 66037 Database links: Entrez Gene: 66037 Human Entrez Gene: 75388 Mouse Omim: 606165 Human SwissProt: Q8N9W6 Human SwissProt: Q924M5 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
