[IF=7.58] Qu, Xinli, et al. "Resolvins E1 and D1 inhibit interstitial fibrosis in the obstructed kidney via inhibition of local fibroblast proliferation." The Journal of pathology 228.4 (2012): 506-519.  WB, IHC-F ;  Mouse.  

PubMed:22610993

[IF=5.195] Jing-Xiao Wang. et al. Chemerin-9 in paraventricular nucleus increases sympathetic outflow and blood pressure via glutamate receptor-mediated ROS generation. EUR J PHARMACOL. 2022 Dec;936:175343  WB ;  Rat.  

PubMed:36306926

[IF=3.61] Peng, Lei, et al. "The Chemerin Receptor CMKLR1 is a Functional Receptor for Amyloid-β Peptide." Journal of Alzheimers Disease (2014).  Mouse.  

PubMed:25079809

[IF=3.448] Hanthazi A et al. Chemerin influences endothelin-and serotonin-induced pulmonary artery vasoconstriction in rats. Life Sci. 2019 Jun 16:116580.  IHC-P ;  Rat.  

PubMed:31216440

Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Liver (Mouse) Lysate at 40 ug
Primary: Anti-CMKLR1 (bs-10185R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CMKLR1 Polyclonal Antibody, Unconjugated(bs-10185R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CMKLR1 Polyclonal Antibody, Unconjugated(bs-10185R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Black line : Positive blank control HUVEC); Negative blank control (A431）
Green line : Primary Antibody (Rabbit Anti-CMKLR1 antibody (bs-10185R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488)
HUVEC（Positive）and A431(Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CMKLR1 Antibody(bs-10185R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).