| 产品编号 | bs-12524R |
| 英文名称 | Rabbit Anti-ARP2 antibody |
| 中文名称 | 肌动蛋白相关蛋白2/3抗体 |
| 别 名 | Actin like protein 2; Actin related protein 2; Actin-like protein 2; ACTIN-RELATED PROTEIN 2; ACTR2; ARP2 actin related protein 2 homolog (yeast); ARP2; ARP2_HUMAN; OTTHUMP00000159937; OTTHUMP00000202270. |
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Specific References (5) | bs-12524R has been referenced in 5 publications.
[IF=6.639] Miran Rada. et al. Cancer Cells Promote Phenotypic Alterations in Hepatocytes at the Edge of Cancer Cell Nests to Facilitate Vessel Co-Option Establishment in Colorectal Cancer Liver Metastases. Cancers. 2022 Jan;14(5):1318 IHC ; Human.
[IF=6.639] Miran Rada. et al. Angiopoietin-1 Upregulates Cancer Cell Motility in Colorectal Cancer Liver Metastases through Actin-Related Protein 2/3. CANCERS. 2022 Jan;14(10):2540 IHC,IF ; Human,Mouse.
[IF=6.268] Rada, Miran. et al. Runt related transcription factor-1 plays a central role in vessel co-option of colorectal cancer liver metastases. Commun Biol. 2021 Aug;4(1):1-15 IHC ; Human.
[IF=4.014] Nuo Heng. et al. RhoA improves cryopreservation of rooster sperm through the Rho/RhoA-associated Kinase/cofilin pathway. POULTRY SCIENCE. 2022 Jul;:102051 WB ; Chicken.
[IF=3.231] Meng-Hao Pan. et al. The Impact of Arp2/3 Complex Inhibition on Cytoskeleton Dynamics and Mitochondrial Function during Goat Oocyte Meiosis. ANIMALS. 2023 Jan;13(2):263 IF ; Goat.
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| 研究领域 | 肿瘤 细胞生物 信号转导 细胞粘附分子 细胞分化 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Pig,Cow,Chicken, Orangutan) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 45kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ARP2/ARP3: 301-394/394 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping of existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia. Function: Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Subunit: Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Subcellular Location: Cytoplasm; cytoskeleton. Cell projection. Similarity: Belongs to the actin family. ARP2 subfamily. SWISS: P61160 Gene ID: 10097 Database links: Entrez Gene: 10097 Human Entrez Gene: 66713 Mouse Entrez Gene: 396147 Chicken Omim: 604221 Human SwissProt: P53488 Chicken SwissProt: P61160 Human SwissProt: P61161 Mouse Unigene: 643727 Human Unigene: 259045 Mouse Unigene: 102249 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ARP2) Polyclonal Antibody, Unconjugated (bs-12524R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
