| 产品编号 | bs-12485R |
| 英文名称 | APC2 |
| 中文名称 | 腺瘤性结肠息肉病蛋白2抗体 |
| 别 名 | Adenomatous polyposis coli protein 2; Adenomatous polyposis coli protein-like; APCL; APC2_HUMAN. |
| 研究领域 | 肿瘤 免疫学 细胞粘附分子 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Dog,Pig,Horse) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 244kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human APCL/APC2: 1-100/2303 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner’s syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS. APC has been found to be associated with structural components of intracellular junctions. b-catenin and g-catenin (also called plakoglobin) are involved in the regulation of cellular adhesion. APC and E cadherin compete for binding to specific internal regions of both b- and g-catenin. Interactions between cytoskeleton and the APC, E cadherin, b/g catenin complex are mediated by a-catenin. Function: APC2 might function in the Wnt signaling pathway through it's ability to promote the rapid degradation of beta-catenin. It also functions as a tumour suppressor. Subunit: Interacts with PSRC1 (By similarity). Interacts with APC, CTNNB1, MAPRE1, MAPRE3, TP53BP2 and possibly with AXIN2. Subcellular Location: Cell Membrane, Cytoplasmic and Golgi Apparatus Tissue Specificity: Widely expressed (at protein level). Specifically expressed in the CNS. Similarity: Belongs to the adenomatous polyposis coli (APC) family. Contains 6 ARM repeats. SWISS: Q9UJX6 Gene ID: 29882 Database links: Entrez Gene: 29882 Human Entrez Gene: 99152 Mouse Omim: 606946 Human SwissProt: Q9UJX6 Human SwissProt: Q8BZQ7 Mouse Unigene: 434898 Human Unigene: 533262 Human Unigene: 291624 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
