| 产品编号 | bs-12400R |
| 英文名称 | NKAP Rabbit pAb |
| 中文名称 | NFKB激活蛋白抗体 |
| 别 名 | NF-kappa-B-activating protein; Nkap; NKAP_HUMAN; Nuclear NF kappaB activating protein; Nuclear NF kappa B activating protein; NFkB activating protein. |
| 研究领域 | 细胞生物 染色质和核信号 信号转导 干细胞 转录调节因子 淋巴细胞 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Sheep,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 47 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NKAP: 321-415/415 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
NFκB, a pleiotropic transcription factor, is present in almost all cell types and is involved in many biological processes including inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFκB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFκB1/p105, NFκB1/p50, REL and NFκB2/p52. This complex is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NKAP (NF-κ-B-activating protein) is a 415 amino acid nuclear protein that regulates TNF and IL1-induced NFκB activation. As component of a DNA-binding complex, NKAP also functions as a transcriptional repressor that acts on NOTCH target genes. Loss of NKAP blocks the development of αβT- cells, suggesting that it is required for their maturation through repression of NOTCH genes. Function: Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter. Subunit: Component of the Notch corepressor complex. Interacts with CIR1 and HDAC3. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the UPF0396 family. SWISS: Q8N5F7 Gene ID: 79576 Database links: Entrez Gene: 79576 Human Omim: 300766 Human SwissProt: Q8N5F7 Human Unigene: 522771 Human |
| 产品图片 |
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal g
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
