| 产品编号 | bs-12377R |
| 英文名称 | Rabbit Anti-OSR1 + OSR2 antibody |
| 中文名称 | OSR1/OSR2蛋白抗体 |
| 别 名 | FLJ90037; Odd skipped related 2; Odd skipped related 2A protein; Odd-skipped related; odd-skipped related 2 (Drosophila); Osr2; OSR2_HUMAN; Protein odd-skipped-related 2; ODD; Odd skipped Drosophila homolog; Odd skipped homolog; Odd skipped related 1 (Drosophila); Odd skipped related 1; Odd skipped related 1 Drosophila; Odd1; Protein odd skipped related 1; OSR1_HUMAN. |
| 研究领域 | 细胞生物 发育生物学 干细胞 锌指蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog,Danio rerio,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OSR2 and OSR1: 181-280/312 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
OSR2 is a 312 amino acid protein that contains five zinc finger domains. It is expressed in the kidneys, skeletal muscle, testis, and mouse embryos and may be involved in transcriptional activity and osteoblast function. The expression of OSR2 is regulated by C/EBP regulatory elements. OSR2 plays a role in regulating palatal development and expression of alkaline phosphatase. Two isoforms, OSR2A and OSR2B, are produced due to alternative splicing. OSR2B is 36 amino acids shorter than OSR2A and contains only three zinc finger motifs. Both isoforms localize to the nucleus and are thought to exhibit opposite transcriptional activities. Mutations in the gene encoding OSR2 can alter the gene expression of Pax-9 and TGF beta3. Subcellular Location: Nucleus. Similarity: Belongs to the Odd C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers. SWISS: Q8TAX0 Q8N2R0 Gene ID: 130497 116039 Database links: Entrez Gene: 130497 Human Entrez Gene: 23967 Mouse Omim: 608891 Human SwissProt: Q8TAX0 Human SwissProt: Q9WVG7 Mouse Entrez Gene: 116039 Human Entrez Gene: 107587 Mouse Omim: 611297 Human SwissProt: Q8N2R0 Human SwissProt: Q91ZD1 Mouse Unigene: 253247 Human Unigene: 46336 Mouse Unigene: 13228 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (OSR1 + OSR2) Polyclonal Antibody, Unconjugated (bs-12377R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse embryo tissue ; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-OSR2 Polyclonal Antibody, Unconjugated(bs-12377R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OSR1 + OSR2) Polyclonal Antibody, Unconjugated (bs-12377R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
