| 产品编号 | bs-12343R |
| 英文名称 | Rabbit Anti-TRIB1 antibody |
| 中文名称 | G蛋白偶联诱导蛋白2受体抗体 |
| 别 名 | Tribbles homolog 1; TRB-1; G-protein-coupled receptor-induced gene 2 protein; GIG-2; SKIP1; TRIB1_HUMAN; C8FW; GIG2; TRB1; Trib1; Tribbles homolog 1. |
| 研究领域 | 发育生物学 信号转导 干细胞 细胞凋亡 激酶和磷酸酶 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Horse,Monkey) |
| 产品应用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 41kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human TRIB1/GIG2: 151-250/372 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
TRB-1 is a 372 amino acid protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase superfamily. Expressed ubiquitously with highest expression in bone marrow, thyroid gland, skeletal muscle and pancreas, TRB-1 interacts with MAPK kinases and is thought to regulate the activation of MAP kinses, possibly controlling MAP kinase cascades. The gene encoding TRB-1 maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies. Function: Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity. Subcellular Location: cytoplasm. Tissue Specificity: Expressed in most human tissues with the highest levels in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily. Contains 1 protein kinase domain. SWISS: Q96RU8 Gene ID: 10221 Database links: Entrez Gene: 10221 Human Entrez Gene: 211770 Mouse Omim: 609461 Human SwissProt: Q96RU8 Human SwissProt: Q8K4K4 Mouse Unigene: 444947 Human Unigene: 40298 Mouse Unigene: 204391 Rat Trib1在脂肪组织维持和脂质代谢紊乱上发挥关键作用; 巨噬细胞至少有两个亚类，即M1型和M2型。M1型巨噬细胞参与促炎反应，且在宿主防御细菌和病毒感染中发挥核心作用。M2巨噬细胞与抗炎反应、寄生虫感染、组织重构、纤维化以及肿瘤疾病发展相关。 Trib1是个衔接蛋白，与泛素连接酶COP1相互作用参与蛋白降解。人类全基因组关联研究表明TRIB1参与脂质代谢。 日前，大阪大学WPI免疫前沿研究中心等处的研究人员，发现Trib1对F4/80 + MR +组织内在巨噬细胞的分化至关重要，且这些巨噬细胞和M2巨噬细胞、酸性粒细胞具有共同的特性(称之为M2类巨噬细胞)。但是，Trib1在M1髓系细胞就不会发挥这种作用。相关研究成果刊登在近期出版的《自然》（Nature）杂志上。 Trib1缺陷导致各种器官中M2类巨噬细胞急剧减少，如骨髓、脾脏、肺以及脂肪组织。在Trib1缺失的骨髓细胞中，C / EBP α的异常表达要为巨噬细胞分化缺陷负责。没有想到的是，造血细胞缺乏 Trib1的小鼠，减少了脂肪组织块，并伴随着脂肪分解增加，即使是在正常饮食条件下。 M2类巨噬细胞的补充可缓解这种病理学现象。这表明，这类巨噬细胞的缺乏是引发脂肪分解的原因。为了应对高脂肪饮食，造血细胞缺乏 Trib1的小鼠发育中会呈现高甘油三酯血症、胰岛素抗性，同时增加促炎细胞基因诱导。 这些结果表明Trib1在脂肪组织维持和脂质代谢紊乱上发挥关键作用，而其作用是通过控制组织内在M2类巨噬细胞的分化实现的。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
