产品编号 | bs-12282R |
英文名称 | CEP135 Rabbit pAb |
中文名称 | 中心体蛋白135抗体 |
别 名 | centrosomal protein 135 kDa; centrosomal protein 135kDa; Centrosomal protein 4; Centrosomal protein of 135 kDa; centrosome protein 4; centrosome protein cep135; Cep135; Cep135; CEP4; CP135_HUMAN. |
研究领域 | 细胞生物 细胞类型标志物 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Dog,Horse) |
产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 133 kDa |
检测分子量 | |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CEP135: 1001-1100/1140 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. Function: Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. Subunit: Interacts with DCTN2 (By similarity). Interacts with CEP250. Subcellular Location: Cytoplasm, cytoskeleton, centrosome, centriole. Note=During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles. DISEASE: Defects in CEP135 are the cause of microcephaly, primary, type 8 (MCPH8) [MIM:614673]. MCPH8 is a disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Affected individuals are mentally retarded. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits due to degenerative brain disorder. Similarity: Belongs to the CEP135/TSGA10 family. SWISS: Q66GS9 Gene ID: 9662 Database links: Entrez Gene: 9662 Human Entrez Gene: 381644 Mouse Omim: 611423 Human SwissProt: Q66GS9 Human SwissProt: Q6P5D4 Mouse Unigene: 518767 Human Unigene: 332452 Mouse |
产品图片 |
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-CEP135 (bs-12282R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 133 kD
Observed band size: 133 kD
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |