| 产品编号 | bs-12244R |
| 英文名称 | Rabbit Anti-HOXA13 antibody |
| 中文名称 | 同源异型盒基因HOXA13抗体 |
| 别 名 | Homeo box 1J; Homeo box A13; Homeobox 1J; Homeobox A13; Homeobox protein Hox A13; Homeobox protein Hox-1J; Homeobox protein Hox-A13; Homeobox protein HOXA13; Homeobox1J; HomeoboxA13; HOX 1; HOX 1J; Hox 1J protein; HOX A13; HOX1; HOX1J; HOXA 13; HOXA13; HXA13_HUMAN; Transcription factor HOXA13. |
| 研究领域 | 发育生物学 干细胞 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Chicken,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human HOXA13: 332-388/388 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Hox proteins play a role in development and cellular differentiation by regulating downstream target genes. Specifically, the Hox proteins direct DNA-protein and protein-protein interactions that assist in determining the morphologic features associated with the anterior-posterior body axis. HoxA13 and HoxD13 also bind to other BMP and TGF-beta/Activin-regulated Smad proteins including Smad1 and Smad2, but not Smad4. In humans and mice, loss of HOXA13 function causes defects in the growth and patterning of the digits and interdigital tissues. Analysis of HoxA13 expression reveals a pattern of localization overlapping with sites of reduced Bmp2 and Bmp7 expression in HoxA13 mutant limbs. HoxA13 regulates Bmp2 and Bmp7 expression, providing a link between HoxA13, its target genes and the specific developmental processes affected by loss of HoxA13 function. Function: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Subcellular Location: Nucleus. DISEASE: Defects in HOXA13 are the cause of hand-foot-genital syndrome (HFGS) [MIM:140000]; also known as hand-foot-uterus syndrome. The clinical features include small feet with unusually short great toes and abnormal thumbs. Females with the disorder have duplication of the genital tract. Defects in HOXA13 are the cause of Guttmacher syndrome (GUTTS) [MIM:176305]. Guttmacher syndrome is a dominantly inherited combination of distal limb and genital tract abnormalities. It has several features in common with hand-foot-genital syndrome, including hypoplastic first digits and hypospadias. Typical features not seen in hand-foot-genital syndrome include postaxial polydactyly of the hands and uniphalangeal second toes with absent nails. Similarity: Belongs to the Abd-B homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: P31271 Gene ID: 3209 Database links: Entrez Gene: 3209 Human Entrez Gene: 15398 Mouse Omim: 142959 Human SwissProt: P31271 Human SwissProt: Q62424 Mouse |
| 产品图片 |
Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug
Lane 2: Uterus (Mouse) Lysate at 40 ug
Lane 3: Liver (Rat) Lysate at 40 ug
Lane 4: Uterus (Rat) Lysate at 40 ug
Lane 5: 293T (Human) Cell Lysate at 30 ug
Lane 6: Hela (Human) Cell Lysate at 30 ug
Lane 7: U2os (Human) Cell Lysate at 30 ug
Primary: Anti-HOXA13 (bs-12244R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 46 kD
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HOXA13 Polyclonal Antibody, Unconjugated(bs-12244R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
