| 产品编号 | bs-10014R |
| 英文名称 | AVPR2 Rabbit pAb |
| 中文名称 | 精氨酸加压素受体2抗体 |
| 别 名 | arginine vasopressin receptor 2; ADHR; Antidiuretic hormone recepto; Arginine vasopressin receptor 2; AVP R2; AVPR 2; AVPR V2; DI1; DIR 3; DIR; DIR3; MGC126533; MGC138386; NDI; Nephrogenic diabetes insipidus; Renal type arginine vasopressin recepto; V2R; Vasopressin V2 receptor. |
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Specific References (1) | bs-10014R has been referenced in 1 publications.
[IF=4.012] Nagano H et al. Muscarinic M receptor promotes vasopressin synthesis in mice supraoptic nuclei. J Endocrinol. 2018 May;237(2):207-216. IHC-P ; Mouse.
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| 研究领域 | 细胞生物 生长因子和激素 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AVPR2: 65-170/371 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
AVPR2 is a receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. AVPR2 is expressed in the kidney tubule, predominantly in the distal convoluted tubule and collecting ducts. The AVPR2 receptor is also expressed outside the kidney although its tissue localization is uncertain. Function: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Kidney. Similarity: Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. SWISS: P30518 Gene ID: 554 Database links: Entrez Gene: 554 Human Entrez Gene: 12000 Mouse Omim: 300538 Human SwissProt: P30518 Human SwissProt: O88721 Mouse Unigene: 567240 Human Unigene: 383171 Mouse Unigene: 209041 Rat |
| 产品图片 |
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-AVPR2 (bs-10014R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 50 kD
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-AVPR2 antibody (bs-10014R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
