| 产品编号 | bs-12050R |
| 英文名称 | 5HT2B Receptor Rabbit pAb |
| 中文名称 | 5-羟色胺受体2B抗体 |
| 别 名 | SR-2B; 5 HT 2B; 5 HT 2B receptor; 5 HT(2B); 5 HT2B; 5 hydroxytryptamine(serotonin) receptor 2B; 5 hydroxytryptamine(serotonin) receptor 2B G protein coupled; 5 hydroxytryptamine 2B receptor; 5 hydroxytryptamine receptor 2B; 5-HT-2B; 5-HT2B; 5-hydroxytryptamine receptor 2B; 5HT(2B); 5HT2B_HUMAN; 5HT2F; Htr 2b; Htr2b; NP75 protein; SEROTONIN 5HT2B RECEPTOR; Serotonin receptor 2B; Stomach fundus serotonin receptor. |
| 研究领域 | 神经生物学 细胞膜受体 G蛋白偶联受体 新陈代谢 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human 5HT2B Receptor/SR-2B: 1-100/481 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Serotonin (also designated 5-hydroxytryptamine or 5-HT) is a molecule that functions as a neurotransmitter, a hormone and a mitogen, and it is predominantly expressed in the gut, platelets and central nervous system (CNS). In the CNS, serotonin modulates several processes, including anxiety, sleep, appetite, behavior and drug abuse. In platelets and gut, serotonin plays a major role in cardiovascular function and motility of the gastrointestinal tract, respectively. Serotonin mediates its effects through several of G protein coupled receptors, designated 5-HT receptors or alternatively SR receptors. The SR-2 receptors are comprised of three subtypes, SR-2A, SR-2B and SR-2C, which activate phospholipase C and release intracellular stores of calcium in response to serotonin. SR-2A has a specific role in tracheal smooth muscle contraction, bronchoconstriction and mediating aldosterone production, and it is also thought to play a role in several psychiatric disorders, including depression and schizophrenia. SR-2B is expressed in embryonic and adult cardiovascular tissues, gut and brain and plays an important role in the pathology of cardiac disorders. SR-2C is thought to mediate the effects of atypical antipsychotic drugs. Function: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Plays a role in the regulation of impulsive behavior. Subunit: Interacts with MPDZ. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in brain. Higher levels of expression are observed in the cerebellum, occipital cortex and frontal cortex. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P41595 Gene ID: 3357 Database links: Entrez Gene: 3357 Human Omim: 601122 Human SwissProt: P41595 Human Unigene: 421649 Human |
| 产品图片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human SH-SY5Y cell lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human HeLa cell lysates
Primary: Anti-5HT2B Receptor (bs-12050R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/2
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
