| 产品编号 | bs-12041R |
| 英文名称 | TAGLN3 Rabbit pAb |
| 中文名称 | 神经元蛋白22抗体 |
| 别 名 | Neuronal protein 22; Neuronal protein NP25; NP22; NP25; TAGL3_HUMAN; TAGLN3; Transgelin 3; Transgelin-3. |
| 研究领域 | 神经生物学 干细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Pig,Sheep,Cow,Chicken,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TAGLN3/NP22: 8-110/199 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Transgelin is a homolog of transgelin and is also expressed in smooth muscle cells and by peritoneal B-1 cells. Unlike the other two transgelin proteins, transgelin-3 (also designated TAGLN2, NP22 (neuronal protein 22) or NP25) is predominantly expressed in brain. Transgelin-3 contains a putative Actin-binding domain, two EF-hand motifs, two potential phosphorylation sites and a calponin-homology (CH) domain. Transgelin-3 shares homology with transgelin and Calponin, two cytoskeleton-interacting proteins. Belonging to the calponin family, transgelin-3 colocalizes with Actin and Tubulin, suggesting a possible role for transgelin-3 in neuronal plasticity or as a signaling protein. Due to a varied expression pattern, transgelin-3 may play different roles in the developing and adult brain. Expression of transgelin-3 is upregulated in regions of the human alcoholic brain. Tissue Specificity: Widely expressed in the brain. Expression is increased in the superior frontal cortex of alcoholics, but not in the motor cortex or cerebellum. Similarity: Belongs to the calponin family. Contains 1 calponin-like repeat. Contains 1 CH (calponin-homology) domain. SWISS: Q9UI15 Gene ID: 29114 Database links: Entrez Gene: 29114 Human Entrez Gene: 56370 Mouse Omim: 607953 Human SwissProt: Q9UI15 Human SwissProt: Q9R1Q8 Mouse Unigene: 169330 Human Unigene: 24183 Mouse Unigene: 10998 Rat |
| 产品图片 |
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TAGLN3 Polyclonal Antibody, Unconjugated(bs-12041R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
