| 产品编号 | bs-11943R |
| 英文名称 | RFX4 Rabbit pAb |
| 中文名称 | 转录调控因子RFX4抗体 |
| 别 名 | NYD SP10; Regulatory factor X 4; Regulatory factor X, 4(influences HLA class II expression); Regulatory factor X4 isoform E; Regulatory factor X4 isoform F; Regulatory factor X4, isoform c; Regulatory factor X4, isoform d; Transcription factor RFX4; Winged helix transcription factor RFX4 variant 3; RFX4_HUMAN. |
| 研究领域 | 细胞生物 免疫学 神经生物学 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Zebrafish,Chicken,Dog,GuineaPig,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 83 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RFX4: /101-200/735 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
EP and EP-like sites are regulatory enhancer elements found in the promoters of several viral and mammalian genes which, in humans, include the MIF-1 binding site (MIE) of the c-Myc gene, the X box of MHC class II promoters and a binding site in the PCNA (proliferating cell nuclear antigen) promoter. The EP-like sites present in the X box of MHC class II promoters are distinctly nonpalindromic sequences that contain only a single EP-homologous half-site. The EP-like element is bound by a ubiquitous nuclear protein complex that consists of homo- and heterodimers involving the RFX1, RFX2, RFX3, RFX4 and RFX5 proteins. The RFX proteins represent an essential class II transcription factor family that share several conserved regions, including a centrally located DNA-binding domain (DBD) and a C-terminal D region that facilitates dimerization. RFX4 is a 735 amino acid nuclear protein that, via interactions with other RFX proteins, can bind DNA and is thought to activate the transcription of target genes. Four isoforms, each of which exhibit different tissue specificity, exist due to alternative splicing events. Function: RFX4 is a transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX4 is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. RFX4 may be a transcriptional repressor rather than a transcriptional activator.This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. This protein may be a transcriptional repressor rather than a transcriptional activator. Three transcript variants encoding different isoforms have been described for this gene. Subunit: Homodimer. Heterodimer with RFX2 and RFX3. Binds DNA. Subcellular Location: Nuclear Tissue Specificity: Isoform 4 is testis-specific. Isoform 1 is expressed in brain and gliomas. Isoform 2 and isoform 3 are testis-specific (at protein level). Isoform 1 is expressed in brain (at protein level). Isoform 3 is expressed at a higher level in adult testes and ejaculated spematozoa than in fetal testes. Similarity: Belongs to the RFX family. Contains 1 RFX-type winged-helix DNA-binding domain. SWISS: Q33E94 Gene ID: 5992 Database links: Entrez Gene: 5992 Human Entrez Gene: 71137 Mouse Omim: 603958 Human SwissProt: Q33E94 Human SwissProt: Q7TNK1 Mouse Unigene: 388827 Human Unigene: 79070 Mouse |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RFX4 Polyclonal Antibody, Unconjugated(bs-11943R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
