| 产品编号 | bs-4974R |
| 英文名称 | Glucose 6 Phosphate Dehydrogenase |
| 中文名称 | 葡萄糖-6磷酸脱氢酶抗体 |
| 别 名 | G6PD; G6PD_HUMAN; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; Glucose-6-phosphate 1-dehydrogenase; MET19; POS10; Zwf1p. 6磷酸葡萄糖脱氢酶; |
| 研究领域 | 心血管 细胞生物 信号转导 糖尿病 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Cow,Horse,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 57kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Glucose 6 Phosphate Dehydrogenase: 351-450/515 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Defects in G6PD are the cause of chronic non-spherocytic hemolytic anemia (CNSHA) . Deficiency of G6PD is associated with hemolytic anemia in two different situations. First, in areas in which malaria has been endemic, G6PD-deficiency alleles have reached high frequencies (1% to 50%) and deficient individuals, though essentially asymptomatic in the steady state, have a high risk of acute hemolytic attacks. Secondly, sporadic cases of G6PD deficiency occur at a very low frequencies, and they usually present a more severe phenotype. Several types of CNSHA are recognized. Class-I variants are associated with severe NSHA; class-II have an activity <10% of normal; class-III have an activity of 10% to 60% of normal; class-IV have near normal activity. Function: Produces pentose sugars for nucleic acid synthesis and main producer of NADPH reducing power. Subunit: Homodimer or homotetramer Tissue Specificity: Isoform Long is found in lymphoblasts, granulocytes and sperm. DISEASE: Defects in G6PD are the cause of chronic non-spherocytic hemolytic anemia (CNSHA) [MIM:305900]. Deficiency of G6PD is associated with hemolytic anemia in two different situations. First, in areas in which malaria has been endemic, G6PD-deficiency alleles have reached high frequencies (1% to 50%) and deficient individuals, though essentially asymptomatic in the steady state, have a high risk of acute hemolytic attacks. Secondly, sporadic cases of G6PD deficiency occur at a very low frequencies, and they usually present a more severe phenotype. Several types of CNSHA are recognized. Class-I variants are associated with severe NSHA; class-II have an activity <10% of normal; class-III have an activity of 10% to 60% of normal; class-IV have near normal activity. Similarity: Belongs to the glucose-6-phosphate dehydrogenase family. SWISS: P11413 Gene ID: 2539 Database links: Entrez Gene: 2539 Human Entrez Gene: 14381 Mouse Entrez Gene: 443211 Sheep Omim: 305900 Human SwissProt: P11413 Human SwissProt: Q00612 Mouse Unigene: 461047 Human Unigene: 684904 Human Unigene: 27210 Mouse Unigene: 11040 Rat |
| 产品图片 |
Sample:
Lymph node (Mouse) Lysate at 40 ug Primary: Anti- Glucose 6 Phosphate Dehydrogenase (bs-4974R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
