| 产品编号 | bs-11880R |
| 英文名称 | Lhx4 Rabbit pAb |
| 中文名称 | Lhx4蛋白抗体 |
| 别 名 | Gsh 4; Gsh4; Lhx4; LHX4_HUMAN; LIM Homeobox 4; LIM homeobox protein 4; LIM/homeobox protein Lhx4. |
| 研究领域 | 细胞生物 神经生物学 信号转导 锌指蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,GuineaPig,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Lhx4: 171-280/390 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The LIM domain (a zinc finger structure) is a protein-protein interaction motif found in several protein types, including homeodomain transcription factors and kinases, which has a role in many cellular processes. The LIM family of homeodomain proteins plays a role in organismal differentiation and development. Specifically, LHX4 and closely related LHX3 play essential roles in multiple developmental stages of the pituitary gland in mice. The LHX4 gene is expressed in murine fetal brain, spinal cord and cerebral cortex. In addition, LHX4 is expressed in the cerebral cortex and in the motor neurons of the CNS in adult rodents. A specific murine LHX4 gene mutation results in a short stature phenotype, pituitary and cerebelllar defects and sella turcica malformations. The LHX4 gene may be implicated in the t(1;4)(q25;q32) chromosomal translocation, which is associated with acute lymphoblastic leukemia. The LHX4 gene is also expressed in leukemic cells and may activate leukemogenesis. The human LHX4 gene maps to chromosome 1q25 and encodes a 390 amino acid protein. Function: May play a critical role in the development of respiratory control mechanisms and in the normal growth and maturation of the lung. Subcellular Location: Nucleus. DISEASE: Defects in LHX4 are the cause of pituitary hormone deficiency combined type 4 (CPHD4) [MIM:262700]; also known as short stature pituitary and cerebellar defects and small sella turcica. The disorder is characterized by short stature, pituitary and cerebellar defects, and small transverse depression crossing the midline on the superior surface of the body of the sphenoid bone which houses the pituitary gland. Similarity: Contains 1 homeobox DNA-binding domain. Contains 2 LIM zinc-binding domains. SWISS: Q969G2 Gene ID: 89884 Database links: Entrez Gene: 89884 Human Entrez Gene: 16872 Mouse Omim: 602146 Human SwissProt: Q969G2 Human SwissProt: P53776 Mouse Unigene: 658487 Human Unigene: 103624 Mouse
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
