| 产品编号 | bs-11831R |
| 英文名称 | Chordin Rabbit pAb |
| 中文名称 | 原肠胚双向形成相关蛋白抗体 |
| 别 名 | Chordin; Chrd; CHRD_HUMAN; MGC133038; Shortgastrulation protein; SOG. |
 |
Specific References (1) | bs-11831R has been referenced in 1 publications.
[IF=7.815] Zhang Y et al. Directed Differentiation of Notochord-like and Nucleus Pulposus-like Cells Using Human Pluripotent Stem Cells. Cell Rep. 2020 Feb 25;30(8):2791-2806.e5. WB ; human.
|
| 研究领域 | 细胞生物 发育生物学 神经生物学 干细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 99 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Chordin: 412-490/951 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The patterning of the CNS relies on the interaction of multiple signaling molecules such as Sonic Hedgehog, Wnts, and BMPs and their antagonists Chordin and Noggin. At midgastrula, expression of Noggin overlaps that of Chordin. Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. The gene which encodeschordin maps to human chromosome 3q27. Noggin is a secreted protein that binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily of signaling proteins, such as BMP-2,4,7. The gene which encodes noggin maps to human chromosome 17q22. Function: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. Subunit: Interacts with TWSG1 and/or BMP4 Subcellular Location: Secreted. Tissue Specificity: Expressed at the highest level in liver. Post-translational modifications: Cleaved by tolloid proteases; cleavage participates to dorsoventral patterning during early development. Similarity: Belongs to the chordin family. Contains 4 CHRD domains. Contains 4 VWFC domains. SWISS: Q9H2X0 Gene ID: 8646 Database links: Entrez Gene: 8646 Human Entrez Gene: 12667 Mouse Omim: 603475 Human SwissProt: Q9H2X0 Human SwissProt: Q9Z0E2 Mouse Unigene: 166186 Human Unigene: 20457 Mouse Unigene: 29459 Rat |
| 产品图片 |
Sample:
HepG2(Human) Cell Lysate at 30 ug
U2OS(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Chordin (bs-11831R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilu
Sample:
HepG2(Human) Cell Lysate at 30 ug
U2OS(Human) Cell Lysate at 30 ug
MDA-MB-231(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Chordin (bs-11831R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/200
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
