| 产品编号 | bs-11755R |
| 英文名称 | SRSF7 Rabbit pAb |
| 中文名称 | 丝氨酸/苏氨酸蛋白激酶SRPK7抗体 |
| 别 名 | 21 GARP; 21 glutamic acid rich protein; 21-GARP; SH3 domain binding glutamic acid rich protein; SH3BGR; SH3BGR protein; Serine/arginine-rich splicing factor 7; SRSF7_HUMAN. |
| 研究领域 | 细胞生物 神经生物学 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Cow,Zebrafish,Chicken) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 27 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SRSF7: 2-70/238 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns, and they are required for accurate splice site recognition and the control of alternative splicing (1). Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF (2). The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/serine (RS)-rich domain, and they are essential for constitutive splicing and also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites (3,4). The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport (5). The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing-sites (6). Function: Splicing factor, arginine/serine rich 7 is required for pre mRNA splicing. Can also modulate alternative splicing in vitro. Subunit: Found in large molecular weight complexes containingCCNL1 and the p110 isoforms of either CDC2L1 or CDC2L2. Interactswith CCNL2 and CPSF6. Subcellular Location: Nuclear Tissue Specificity: Brain, liver, kidney and lung. Post-translational modifications: Extensively phosphorylated on serine residues in the RSdomain. Similarity: Belongs to the splicing factor SR family. Contains 1 CCHC-type zinc finger. Contains 1 RRM (RNA recognition motif) domain. SWISS: Q16629 Gene ID: 6432 Database links: Entrez Gene: 6432 Human Omim: 600572 Human SwissProt: Q16629 Human Unigene: 309090 Human |
| 产品图片 |
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SFRS7 Polyclonal Antibody, Unconjugated(bs-11755R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
