| 产品编号 | bs-11743R |
| 英文名称 | ELAVL2 + ELAVL4 Rabbit pAb |
| 中文名称 | ELAVL2+ELAVL4蛋白抗体 |
| 别 名 | ELAV like 4; ELAV like neuronal protein 1; ELAV like protein 2; ELAV like protein 4; ELAVL 2; ELAVL 4; HELN1; Hu antigen B; Hu antigen D; HUB; HUD; ELAV2_HUMAN; ELAV4_HUMAN. |
| 研究领域 | 细胞生物 神经生物学 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Cow,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 39+42 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ELAVL2: 31-110/359 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Elav-like genes encode for a family of RNA-binding proteins. Elav, a Drosophila protein and the first described member, is expressed immediately after neuroblastic differentiation into neurons and is necessary for neuronal differentiation and maintenance. Several mammalian Elav-like proteins, designated HuC, HuD and Hel-N1, are also expressed in postmitotic neurons. An additional mammalian homolog, HuR, which is also designated HuA, is ubiquitously expressed and is also overexpressed in a wide variety of tumors. Characteristically, these homologs all contain three RNA recognition motifs (RRM) and they specifically bind to AU-rich elements (ARE) in the 3'-untranslated region of mRNAs transcripts. ARE sites target mRNA for rapid degradation and thereby regulate the expression levels of genes involved in cell growth and differentiation. When Elav-like proteins associate with these ARE sites this degradation is inhibited, leading to an increased stability of the corresponding transcript. Elav proteins function within the nucleus, and they are shuttled between the nucleus and cytoplasm by a nuclear export signal, which is a regulatory feature of the Elav-like proteins as it limits their accessibility to ARE sites. Function: ELAV2 binds RNA and seems to recognize a GAAA motif and is neuronal specific. ELAVL4 may play a role in neuron-specific RNA processing. It contains 3 RNA recognition motif (RRM) domains and is localised to brain tissue. ELAVL4 has been linked with the disease, paraneoplastic encephalomyelitis sensory neuropathy, which is associated with small cell lung cancer and is characterized by dementia, sensory loss, and other neurological disabilities. This disease may result from an immune response primarily directed against a small cell lung tumor antigen which is misdirected against similar antigens expressed in brain (such as ELAVL4). Subcellular Location: Interacts with IGF2BP1. Tissue Specificity: Brain; neural-specific. Similarity: Belongs to the RRM elav family. Contains 3 RRM (RNA recognition motif) domains. SWISS: P26378.2 Gene ID: 1996 Database links: Entrez Gene: 1993 Human Entrez Gene: 1996 Human Entrez Gene: 15569 Mouse Entrez Gene: 15572 Mouse Omim: 168360 Human Omim: 601673 Human SwissProt: P26378 Human SwissProt: Q12926 Human SwissProt: Q60899 Mouse SwissProt: Q61701 Mouse UniProtKB/Swiss-Prot: Q12926.2 UniProtKB/Swiss-Prot: P26378.2 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELAVL2 + ELAVL4) Polyclonal Antibody, Unconjugated (bs-11743R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
