| 产品编号 | bs-4527R |
| 英文名称 | Rabbit Anti-CSFV E2 antibody |
| 中文名称 | 猪瘟病毒包膜糖蛋白E2抗体 |
| 别 名 | polyprotein [Classical swine fever virus]; polyprotein; CSFV polyprotein; E2; Envelope glycoprotein E2; structural protein E2 [Classical swine fever virus]; POLG_CSFVA. |
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Specific References (1) | bs-4527R has been referenced in 1 publications.
[IF=5.818] Xiaodong Zou. et al. Cholesterol Biosynthesis Modulates CSFV Replication. VIRUSES-BASEL. 2022 Jul;14(7):1450 IF ; Pig.
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| 研究领域 | 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | CSFV |
| 产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from CSFV Envelope glycoprotein E2: 951-1050/3898 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Classical swine fever(CSF) is a kind of acute and intense contagious disease in swine, it material damages swine industry. CSF could cause swine appearing disseminated hemorrhage in systemic organ and tissue, degeneration and necrosis in small vascular and capillary endothelial cells. Classical swine fever virus (CSFV) belongs to genera Pestivirus, family Flaviviridae, it’s genome total length is 12.3Kb,including a big ORF, encoding 11 structural and nonstructural proteins. Envelope glycoprotein E2 exists in the form of homodimer; this protein is cleaved from the polyprotein by a cellular signal peptidase. Function: E(rns), E1 and E2 are responsible of cell attachment and subsequent fusion of viral and cellular membrane. P7 forms a leader sequence to properly orient NS2 in the membrane. Uncleaved NS2-3 is required for production of infectious virus. NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus. NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS4A is a cofactor for the NS3 protease activity. RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome. Subunit: The E(rns) glycoprotein is found as a homodimer; disulfide-linked. The E1 and E2 envelope glycoproteins form disulfide-linked homodimers as well as heterodimers. Subcellular Location: E(rns) glycoprotein: Host membrane; Peripheral membrane protein. Note: The C-terminus membrane anchor of Erns represents an amphipathic helix embedded in plane into the membrane Similarity: Belongs to the pestivirus polyprotein family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 peptidase C53 domain. Contains 1 peptidase C74 domain. Contains 1 peptidase S31 domain. Contains 1 RdRp catalytic domain. SWISS: P21530 Gene ID: 920146 Database links: Entrez Gene: 920146 CSFVA SwissProt: P19712 CSFVA |
| 产品图片 |
Sample:
Lane 1: Recombinant CSFV E2 protein (200ng) (bs-20116P)
Primary: Anti-CSFV E2 (bs-4527R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42kDa
Observed band size: 32kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
