| 产品编号 | bs-11675R |
| 英文名称 | UBXD2 |
| 中文名称 | UBX结构域域蛋白D2抗体 |
| 别 名 | Erasin; FLJ23318; KIAA0242; KIAA2042; UBX domain containing 1; UBX domain containing 2; UBX domain containing protein 2; UBXD 2; UBXDC 1; UBXDC1; UBXN4_HUMAN. |
| 研究领域 | 细胞生物 神经生物学 信号转导 Alzheimer's |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Pig,Cow,Horse,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 57kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human UBXD2: 201-300/508 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Erasin is an endoplasmic reticulum (ER) and nuclear envelope membrane protein. Expressed in a variety of tissues, such as brain, placenta, heart, liver, prostate, kidney, pancreas, lung and skeletal muscle, erasin contains one UBX domain and participates in the clearing of ERAD (endoplasmic reticulum-associated protein degradation) substrates. The UBX domain of erasin is responsible for mediating its direct interaction with VCP (valosin-containing protein), an AAA-ATPase molecular chaperone. In response to ER stress, erasin expression is induced. The knockdown of erasin expression leads to the inhibition of ERAD, suggesting an important function of erasin in the ERAD pathway. In addition, erasin may be involved in Alzheimer’s disease, as it is known to accumulate in neurofibrillary degenerating neurons in patients with Alzheimer’s disease. Function: UBXD2 is an integral membrane protein of the endoplasmic reticulum (ER) that binds valosin-containing protein (VCP) and promotes ER-associated protein degradation (ERAD). Immunohistochemical staining of brain tissue from patients with Alzheimer's disease and control subjects revealed that UBXD2 accumulates preferentially in neurons undergoing neurofibrillary degeneration in Alzheimer's disease. Subunit: Directly interacts with VCP. Subcellular Location: Endoplasmic reticulum membrane. Both the N- and the C-terminus face the cytosol; also found in the nucleus envelope contiguous to the ER. Tissue Specificity: Expressed in many tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Accumulates in Alzheimer disease-afflicted brains (at protein level). Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 UBX domain. SWISS: Q92575 Gene ID: 23190 Database links: Entrez Gene: 23190 Human Entrez Gene: 67812 Mouse Omim: 611216 Human SwissProt: Q92575 Human SwissProt: Q8VCH8 Mouse Unigene: 709238 Human |
| 产品图片 |
Sample:
Heart (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-UBXD2 (bs-11675R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD 
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBXD2) Polyclonal Antibody, Unconjugated (bs-11675R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBXD2) Polyclonal Antibody, Unconjugated (bs-11675R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
